具有卡那霉素抗性标记的新型多功能克隆载体。
New and versatile cloning vectors with kanamycin-resistance marker.
作者信息
Pridmore R D
机构信息
Ciba-Geigy AG, Department of Biotechnology, Basel, Switzerland.
出版信息
Gene. 1987;56(2-3):309-12. doi: 10.1016/0378-1119(87)90149-1.
PMID:3315864
Abstract
Described here is a pair of small multi-copy kanamycin-resistance plasmids, containing the pUC lacZ alpha-complementation peptide and the pUC18 and pUC19 multiple cloning site. These plasmids and their derivatives allow simple and rapid transfer of inserts from one replicon to another without the necessity of purifying the insert from vector.
摘要
这里描述的是一对小型多拷贝卡那霉素抗性质粒，其含有pUC lacZα互补肽以及pUC18和pUC19多克隆位点。这些质粒及其衍生物能够实现将插入片段从一个复制子简单快速地转移至另一个复制子，而无需从载体中纯化插入片段。
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