Rozmiarek H, Bolton R W, Chorpenning F W
Infect Immun. 1977 May;16(2):505-9. doi: 10.1128/iai.16.2.505-509.1977.
In an effort to determine the origin of natural antibodies to teichoic acid, rats were fed a sterile liquid diet free of detectable teichoic acid and virtually free of gram-positive bacteria. Both germ-free and conventional Sprague-Dawley rats raised on this diet failed to produce antibodies to polyglycerophosphate, whereas 100% of their counterparts fed the usual teichoic acid-containing diet did produce these antibodies. The intestinal flora was similar in both groups of animals. When the test animals were immunized intraperitoneally or orally with gram-positive bacteria, 100% displayed immunocompetency by producing significant levels of antibody. These results demonstrate the environmental nature of the antigenic stimulus for these antibodies and suggest the importance of food as the major source of stimulation. The experimental model described here furnishes a valuable tool for studies of immunologic responses where a single known specificity and a controlled system would be advantageous.
为了确定针对磷壁酸的天然抗体的来源，给大鼠喂食不含可检测到的磷壁酸且几乎不含革兰氏阳性菌的无菌液体饮食。以这种饮食饲养的无菌和常规斯普拉格-道利大鼠均未能产生针对聚甘油磷酸的抗体，而喂食通常含磷壁酸饮食的同类大鼠中有100%产生了这些抗体。两组动物的肠道菌群相似。当用革兰氏阳性菌对试验动物进行腹腔内或口服免疫时，100%的动物通过产生显著水平的抗体表现出免疫能力。这些结果证明了这些抗体抗原刺激的环境性质，并表明食物作为主要刺激源的重要性。这里描述的实验模型为免疫反应研究提供了一个有价值的工具，在这种研究中，单一已知特异性和可控系统将是有利的。
