Hughes G, McKerr G, Allen J, Barnett Y
Cancer and Ageing Research Group, School of Biomedical Sciences, University of Ulster, Coleraine, County Londonderry, UK.
Cancer Lett. 1996 Jun 5;103(2):219-25. doi: 10.1016/0304-3835(96)04217-6.
To further investigate the possibility for retroviral involvement in the etiology of human breast cancer we processed peripheral blood monocytes and malignant breast tissue biopsies from 10 patients with breast cancer (infiltrating ductal carcinoma or infiltrating lobular carcinoma; ages 40-80 years) and 20 normal healthy women (with no evidence or family history of breast cancer. 10 age-matched controls and 10 women age 22-27 years) for the assay of the retroviral enzyme, reverse transcriptase, using an ELISA and for election microscopy examination for the detection of retroviral-like particles. Reverse transcriptase activity was detected in 5 out of 10 samples of monocyte culture medium and in 1 out of 10 of malignant tissue biopsies from the patients with breast cancer. In contrast, reverse transcriptase was not detected in the culture medium of the monocytes from any of the control subjects. Electron microscopy did not reveal the presence of any retroviral-like particles in any sample of monocyte culture medium or in any of the malignant or normal breast tissue biopsies. Despite evidence for the presence of reverse transcriptase in a subsample of the monocyte culture medium and breast tissue biopsies from the cohort of breast cancer patients who participated in this study, the role of retroviruses in human breast cancer remains unclear.
为进一步研究逆转录病毒与人类乳腺癌病因的相关性，我们对10例乳腺癌患者（浸润性导管癌或浸润性小叶癌；年龄40 - 80岁）以及20名正常健康女性（无乳腺癌证据或家族史，其中10名年龄匹配的对照者和10名年龄在22 - 27岁的女性）的外周血单核细胞和恶性乳腺组织活检样本进行了处理，采用酶联免疫吸附测定（ELISA）检测逆转录病毒酶——逆转录酶，并通过电子显微镜检查来检测类逆转录病毒颗粒。在10份单核细胞培养基样本中，有5份检测到逆转录酶活性，在10份乳腺癌患者的恶性组织活检样本中，有1份检测到逆转录酶活性。相比之下，在任何对照受试者的单核细胞培养基中均未检测到逆转录酶。电子显微镜检查未在任何单核细胞培养基样本以及任何恶性或正常乳腺组织活检样本中发现类逆转录病毒颗粒。尽管在参与本研究的乳腺癌患者队列的部分单核细胞培养基和乳腺组织活检样本中存在逆转录酶的证据，但逆转录病毒在人类乳腺癌中的作用仍不明确。
