Yahiaoui B, Taibi A, Ouaissi A
Laboratoire de Recherche sur les Trypanosomatidae, INSERM U 415, Institut Pasteur, Lille, France.
Gene. 1996 Feb 22;169(1):115-8. doi: 10.1016/0378-1119(95)00785-7.
We have isolated a cDNA from the protozoan parasite Leishmania major (Lm) that encodes a protein homologous to the Saccharomyces cerevisiae and Kluyveromyces marxianus silent information regulator 2 (SIR2) proteins. The deduced Lm SIR2-related protein (termed LmSIR2rp) consists of 381 amino acids that share 40.5% identity with yeast SIR2, increasing to 60% when substitutions are included. Moreover, the LmSIR2rp aa sequence contains a single potential zinc-binding domain with a CysXaa2CysXaa20CysXaa2Cys motif, and its C-terminal part is rich in Ser (16 Ser residues over 40 aa) which constitute potential sites for phosphorylation. The characterization of a novel Lm gene product which shows considerable similarity to a yeast mating-type regulatory protein provides a new tool to investigate the parasite differentiation control mechanisms and gene expression regulation.
我们从原生动物寄生虫硕大利什曼原虫（Lm）中分离出了一个cDNA，它编码一种与酿酒酵母和马克思克鲁维酵母沉默信息调节因子2（SIR2）蛋白同源的蛋白质。推导的Lm SIR2相关蛋白（称为LmSIR2rp）由381个氨基酸组成，与酵母SIR2的同源性为40.5%，若包括替换情况则增至60%。此外，LmSIR2rp氨基酸序列包含一个单一的潜在锌结合结构域，具有CysXaa2CysXaa20CysXaa2Cys基序，其C末端富含Ser（40个氨基酸中有16个Ser残基），这些Ser残基构成了潜在的磷酸化位点。一种与酵母交配型调节蛋白具有显著相似性的新型Lm基因产物的特性，为研究寄生虫分化控制机制和基因表达调控提供了一种新工具。
