Schlueter A J, Malek T R, Hostetler C N, Smith P A, deVries P, Waldschmidt T J
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA.
J Immunol. 1997 May 1;158(9):4211-22.
The expression patterns of the Ly-6C Ag were examined on splenic and thymic lymphocyte subsets of Ly-6.1 and Ly-6.2 strains of mice using the rat mAb 15.1. Ly-6C is expressed on subsets of CD4+ and CD8+ splenocytes, and a portion of NK cells. Within the splenic and lymph node CD4+ T cell compartment, Ly-6C expression is restricted to Ly-6.2 strains of mice, and is present on a subset of naive cells. Ly-6C is expressed on the majority of peripheral CD8+ T cells in both Ly-6.1 and Ly-6.2 strains, and is found primarily on the Ag-experienced subset. In the thymus, Ly-6C is present on subpopulations of CD4- CD8+, CD4- CD8-, and CD4+ CD8- cells. Ly-6C+ CD4- CD8+ thymocytes show a mature phenotype, while Ly-6C+ CD4- CD8- and Ly-6C+ CD4+ CD8- thymocytes appear to be part of the recently described NK1.1+ alphabeta TCR+ population. On account of the marked differences in Ly-6C expression on peripheral CD4+ T cells from Ly-6.1 and Ly-6.2 strains of mice, additional experiments were undertaken to assess Ly-6C expression in parental and Ly-6.1 x Ly-6.2 F1 mice. Neither phenotype dominated in the F1 offspring, with frequencies of Ly-6C+ CD4+ splenocytes falling in the intermediate range. Further experiments compared the staining patterns of the rat anti-pan Ly-6C (Ly-6.1 and Ly-6.2) Ab with a mouse anti-Ly-6.2 allotype specific Ab, with emphasis on both Ly-6.2 and Ly-6.1 x Ly-6.2 F1 mice. The results demonstrate the presence of lymphocytes that express the pan form of Ly-6C but not the form recognized by the alloantibody. This latter finding suggests the presence of more than one form of the Ly-6C Ag.
使用大鼠单克隆抗体15.1，在Ly-6.1和Ly-6.2品系小鼠的脾脏和胸腺淋巴细胞亚群上检测Ly-6C抗原的表达模式。Ly-6C在CD4⁺和CD8⁺脾细胞亚群以及一部分自然杀伤（NK）细胞上表达。在脾脏和淋巴结的CD4⁺T细胞区室中，Ly-6C的表达仅限于Ly-6.2品系小鼠，且存在于一部分幼稚细胞上。在Ly-6.1和Ly-6.2品系中，Ly-6C在大多数外周CD8⁺T细胞上表达，且主要存在于抗原接触过的亚群中。在胸腺中，Ly-6C存在于CD4⁻CD8⁺、CD4⁻CD8⁻和CD4⁺CD8⁻细胞亚群中。Ly-6C⁺CD4⁻CD8⁺胸腺细胞表现出成熟的表型，而Ly-6C⁺CD4⁻CD8⁻和Ly-6C⁺CD4⁺CD8⁻胸腺细胞似乎是最近描述的NK1.1⁺αβTCR⁺群体的一部分。鉴于Ly-6.1和Ly-6.2品系小鼠外周CD4⁺T细胞上Ly-6C表达存在显著差异，因此进行了额外实验以评估亲本品系和Ly-6.1×Ly-6.2 F1代小鼠中Ly-6C的表达。在F1代后代中，两种表型均未占主导，Ly-6C⁺CD4⁺脾细胞的频率处于中间范围。进一步的实验比较了大鼠抗泛Ly-6C（Ly-6.1和Ly-6.2）抗体与小鼠抗Ly-6.2同种异型特异性抗体的染色模式，重点研究了Ly-6.2和Ly-6.1×Ly-6.2 F1代小鼠。结果表明存在表达泛型Ly-6C但不表达同种异体抗体识别形式的淋巴细胞。后一发现表明存在不止一种形式的Ly-6C抗原。
