牙科技术实验室特定风险区域的微生物学研究。
Microbiological study of selected risk areas in dental technology laboratories.
作者信息
Verran J, Kossar S, McCord J F
机构信息
Department of Biological Sciences, Manchester Metropolitan University, Turner Dental School, University of Manchester, UK.
出版信息
J Dent. 1996 Jan-Mar;24(1-2):77-80. doi: 10.1016/0300-5712(95)00052-6.
OBJECTIVES
To investigate the microbiological status of certain risk areas in the dental technology laboratory, namely pumice slurry, impression agar and curing water baths.
METHODS
Samples were inoculated onto selective and non-selective media. Resultant fungal and bacterial colonies were counted and identified to genus or species level.
RESULTS
Pumice slurry freshly made up using disinfectant was free from contamination, but colony counts approached 10(9) g-1 after 3 days' use. Impression agar was free from contamination, probably because of its method of preparation and maintenance, which appears to achieve sterility. Staphylococci were most commonly isolated from curing water baths (and from the air). Candida spp. and other yeasts were common in pumice slurry.
CONCLUSIONS
Personnel and managers should be aware of the potential cross-contamination hazards posed by the presence of a range of opportunist pathogens in dental technology laboratories.
目的
调查牙科技术实验室中某些风险区域的微生物状况，即浮石糊剂、印模琼脂和固化水浴。
方法
将样本接种到选择性和非选择性培养基上。对产生的真菌和细菌菌落进行计数，并鉴定到属或种水平。
结果
使用消毒剂新配制的浮石糊剂无污染，但使用3天后菌落数接近10⁹ g⁻¹。印模琼脂无污染，可能是由于其制备和保存方法似乎能实现无菌状态。固化水浴（以及空气中）最常分离出葡萄球菌。念珠菌属和其他酵母菌在浮石糊剂中很常见。
结论
工作人员和管理人员应意识到牙科技术实验室中存在一系列机会性病原体所带来的潜在交叉污染风险。
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