Ricci M L, von Hunolstein C, Gomez M J, Parisi L, Tissi L, Orefici G
Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy.
J Med Microbiol. 1996 Jun;44(6):475-81. doi: 10.1099/00222615-44-6-475.
A murine IgM monoclonal antibody (MAb H11) was developed against the type polysaccharide capsular antigen of group B streptococcus (GBS), serotype IV, after intraperitoneal immunisation of BALB/c mice with heat-killed bacteria. MAb H11 reacted in immunodiffusion with the purified polysaccharide in both its sialylated and desialylated form, giving a line of identity, and opsonised type IV GBS strains in an in vitro assay. When administered at the time of intraperitoneal lethal challenge with homologous GBS, or 4 h earlier, MAb H11 protected 90% of the mice. Protection was still observed when MAb H11 was given 4 h after the challenge. This MAb was strongly effective in preventing septic arthritis induced by type IV GBS.
用热灭活的B族链球菌（GBS）血清型IV型多糖荚膜抗原对BALB/c小鼠进行腹腔免疫后，制备了一种抗该抗原的鼠源IgM单克隆抗体（MAb H11）。MAb H11在免疫扩散试验中能与纯化的多糖（无论其唾液酸化形式还是去唾液酸化形式）发生反应，形成一条同一线，并且在体外试验中能调理血清型IV型GBS菌株。当在腹腔内用同源GBS进行致死性攻击时或提前4小时给予MAb H11，可保护90%的小鼠。在攻击后4小时给予MAb H11时，仍可观察到保护作用。该单克隆抗体在预防血清型IV型GBS诱导的化脓性关节炎方面非常有效。
