Barrera J C, Letchworth G J
Department of Animal Health and Biomedical Sciences, University of Wisconsin at Madison 53706, USA.
Virology. 1996 May 15;219(2):453-64. doi: 10.1006/viro.1996.0271.
Persistence of vesicular stomatitis virus New Jersey (VSV-NJ) was studied in experimentally infected hamsters (Mesocricetus auratus). We used reverse transcription and nested polymerase chain reaction (RT-NPCR) to probe tissues of hamsters inoculated with VSV-NJ Hazelhurst. Viral genomic RNA was detected in the brain, cerebellum, spleen, liver, kidney, and lung 2 months after infection, but only in the central nervous system at 10 and 12 months. Viral messenger RNA was detected in the brain of one hamster at 2 months after infection. Replicative intermediate was detected in the spinal cord of one hamster at 12 months. These results suggest that VSV-RNA persists in animals for long periods following infection, disease, and convalescence. However, infectious virus was not recovered from tissues by conventional serial passages of tissue extracts in Vero cells or by cocultivation.
在实验感染的仓鼠（金黄仓鼠）中研究了水疱性口炎病毒新泽西株（VSV-NJ）的持续性。我们使用逆转录和巢式聚合酶链反应（RT-NPCR）来检测接种VSV-NJ Hazelhurst株的仓鼠组织。感染后2个月，在脑、小脑、脾脏、肝脏、肾脏和肺中检测到病毒基因组RNA，但在10个月和12个月时仅在中枢神经系统中检测到。感染后2个月，在一只仓鼠的脑中检测到病毒信使RNA。在一只仓鼠的脊髓中于12个月时检测到复制中间体。这些结果表明，VSV-RNA在感染、发病和康复后的动物体内长期持续存在。然而，通过在Vero细胞中对组织提取物进行常规连续传代或共培养，未从组织中回收感染性病毒。
