Letchworth G J, Barrera J C, Fishel J R, Rodriguez L
Department of Animal Health and Biomedical Sciences, University of Wisconsin at Madison 53706, USA.
Virology. 1996 May 15;219(2):480-4. doi: 10.1006/viro.1996.0275.
To test the hypothesis that vesicular stomatitis New Jersey virus (VSV-NJ) persists in convalescent cattle, we used explant cultures and reverse transcription nested polymerase chain reactions to probe for viral genomic, replicative intermediate, and mRNA in two cows experimentally inoculated in the tongue 5 months earlier and three cows naturally infected 4-14 months previously. Virus was not isolated from any tissues of any animal. Sequences of the viral polymerase and nucleocapsid genes were consistently identified in the tongue and lymph nodes draining the tongue of both experimentally infected animals but not in the three naturally infected animals. Replicative intermediate but not messenger RNA sequences were detected. These results showed for the first time the long term persistence of VSV-NJ RNA in its bovine host.
为了验证水疱性口炎新泽西病毒（VSV-NJ）在康复牛体内持续存在的假说，我们使用外植体培养和逆转录巢式聚合酶链反应，对5个月前经实验接种于舌部的两头奶牛以及4 - 14个月前自然感染的三头奶牛的病毒基因组、复制中间体和mRNA进行检测。未从任何动物的任何组织中分离出病毒。在两只经实验感染动物的舌部以及引流舌部的淋巴结中，始终能鉴定出病毒聚合酶和核衣壳基因的序列，但在三只自然感染动物中未检测到。检测到了复制中间体序列，但未检测到信使RNA序列。这些结果首次表明VSV-NJ RNA在其牛宿主中能长期持续存在。
