Nakamura Y, Tanaka T, Kuwahara Y
Department of Orthodontics, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
Am J Orthod Dentofacial Orthop. 1996 Apr;109(4):348-54. doi: 10.1016/s0889-5406(96)70115-4.
The degenerating tissues found in rat periodontal ligaments during tooth movement were examined morphologically, histochemically, and elementally, with decalcified and unfixed, undecalcified frozen sections. There were two types of degenerating tissues found in the compressed periodontal ligaments: One (type A tissue) was stained differently from collagen and the other (type B tissue) showed the same color as collagen. Type A tissue also showed the deposition of fibrin in Martius scalet blue and Weigert stain. The electron micrograph also showed the deposition of fibrin in type A tissue. No collagen fibers with typical bandings were seen in either tissue. The digestion experiment showed that type A tissue was digested by trypsin but not type B, whereas most of type B tissue was digested by collagenase but not type A. The backscattered electron image by scanning electron microscopy of type A tissue of the unfixed undecalcified frozen sections showed the presence of many small pieces. The elemental analysis of the pieces showed high peaks of phosphorous and calcium. These results indicate that collagen degradation, fibrin deposition, and calcification occurred in the degenerating tissues, especially in type A tissue during the experimental tooth movement.
利用脱钙和未固定、未脱钙的冰冻切片，对大鼠牙齿移动过程中牙周韧带内的退变组织进行了形态学、组织化学和元素分析。在受压的牙周韧带中发现了两种退变组织：一种（A 型组织）染色与胶原不同，另一种（B 型组织）与胶原颜色相同。A 型组织在马休黄猩红蓝染色和魏格特染色中也显示有纤维蛋白沉积。电子显微镜图像也显示 A 型组织中有纤维蛋白沉积。两种组织中均未见有典型条纹的胶原纤维。消化实验表明，A 型组织可被胰蛋白酶消化，而 B 型组织则不能，而大多数 B 型组织可被胶原酶消化，A 型组织则不能。未固定、未脱钙冰冻切片的 A 型组织扫描电子显微镜背散射电子图像显示有许多小碎片。这些碎片的元素分析显示磷和钙有高峰值。这些结果表明，在实验性牙齿移动过程中，退变组织尤其是 A 型组织中发生了胶原降解、纤维蛋白沉积和钙化。
