Kanavaros P, Briere J, Lescs M C, Gaulard P
Department of Pathology, Hopital Henri Mondor, Creteil, France.
J Pathol. 1996 Mar;178(3):297-302. doi: 10.1002/(SICI)1096-9896(199603)178:3<297::AID-PATH469>3.0.CO;2-E.
Fifty-five cases of non-Hodgkin's lymphoma (NHL) of the upper respiratory tract, comprising 27 sinonasal (SN) and 28 Waldeyer's ring (WR) NHL, were investigated for expression of Epstein-Barr virus (EBV)-encoded EBER transcripts and latent membrane protein-1 (LMP-1) by RNA in-situ hybridization (RISH) and immunohistochemistry, respectively. Thirty-two cases were B-cell tumours (10 SNHLs and 22 WRNHLs) and 23 cases expressed natural killer (NK) and/or T-cell antigens (17 SNHLs and 6 WRNHLs). EBER transcripts were detected in tumour cells in 19 lymphomas expressing NK and/or T-cell antigens (16/17 SHNHLs and 3/6 WRNHLs) but in only 2/32 B-NHLs (1/10 SNHLs and 1/22 WRNHLs). LMP-1 expression was found in tumour cells in the 19 EBER-positive tumours expressing NK and/or T-cell antigens but in none of the B-cell lymphomas. All the LMP-1-positive lymphomas expressed the CD30 molecule in tumour cells. These results indicate that in lymphomas of the upper respiratory tract, EBV is strongly associated with sinonasal localization and expression of NK and/or T-cell antigens by tumour cells. EBV can also be detected in some cases of WRNHLs expressing NK and/or T-cell antigens, whereas it is rarely found in B-cell SNHLs and WRNHLs. Furthermore, the detection of the LMP-1 protein in tumour cells in most SNHLs and some WRNHLs expressing NK and/or T-cell antigens, in view of the LMP-1 transforming potential, suggests that EBV may play a role in the pathogenesis of these lymphomas.
对55例上呼吸道非霍奇金淋巴瘤（NHL）进行了研究，其中包括27例鼻窦（SN）NHL和28例韦氏环（WR）NHL，分别通过RNA原位杂交（RISH）和免疫组织化学检测了爱泼斯坦-巴尔病毒（EBV）编码的EBER转录本和潜伏膜蛋白1（LMP-1）的表达。32例为B细胞肿瘤（10例鼻窦NHL和22例韦氏环NHL），23例表达自然杀伤（NK）和/或T细胞抗原（17例鼻窦NHL和6例韦氏环NHL）。在表达NK和/或T细胞抗原的19例淋巴瘤的肿瘤细胞中检测到EBER转录本（16/17例鼻窦NHL和3/6例韦氏环NHL），但在32例B-NHL中仅2例检测到（1/10例鼻窦NHL和1/22例韦氏环NHL）。在表达NK和/或T细胞抗原的19例EBER阳性肿瘤的肿瘤细胞中发现LMP-1表达，但在B细胞淋巴瘤中均未发现。所有LMP-1阳性淋巴瘤的肿瘤细胞均表达CD30分子。这些结果表明，在上呼吸道淋巴瘤中，EBV与鼻窦定位以及肿瘤细胞表达NK和/或T细胞抗原密切相关。在一些表达NK和/或T细胞抗原的韦氏环NHL病例中也可检测到EBV，而在B细胞鼻窦NHL和韦氏环NHL中很少发现。此外，鉴于LMP-1的转化潜能，在大多数鼻窦NHL和一些表达NK和/或T细胞抗原的韦氏环NHL的肿瘤细胞中检测到LMP-1蛋白，提示EBV可能在这些淋巴瘤的发病机制中起作用。
