Nakagawa H, Aihara K, Tsurufuji S
J Biochem. 1977 Mar;81(3):801-4. doi: 10.1093/oxfordjournals.jbchem.a131518.
The crude cathepsin B1 isolated from granulation tissue was separated into two major peaks (P-2 and P-3 fractions) by chromatography on CM-cellulose. The changes in cathepsin B1 activity were studied at various phases of the development of granulation tissue. The cathepsin B1 activity of P-2 fraction, in contrast with that of P-3 fraction, increased with the passage of time after carrageenin injection and the cathepsin B1 activity of P-2 fraction was higher in the resorbing tissues than in non-resorbing tissues, suggesting that cathepsin B1 in P-2 fraction plays an important role in the resorption of granulation tissue, including collagen breakdown.
从肉芽组织中分离出的粗组织蛋白酶B1，通过在CM-纤维素上进行色谱分析，被分离成两个主要峰（P-2和P-3组分）。研究了肉芽组织发育各阶段组织蛋白酶B1活性的变化。与P-3组分相比，P-2组分的组织蛋白酶B1活性在注射角叉菜胶后随时间推移而增加，且P-2组分在吸收组织中的组织蛋白酶B1活性高于非吸收组织，这表明P-2组分中的组织蛋白酶B1在肉芽组织的吸收中起重要作用，包括胶原蛋白的分解。
