Fujihara K, Yamanaka N, Bernstein J M, Ogra P L, Hard R
Department of Otorhinolaryngology, Wakayama Medical College, Japan.
Ann Otol Rhinol Laryngol. 1996 Jun;105(6):452-7. doi: 10.1177/000348949610500606.
We focused on the pathogenicity of otitis media with effusion (OME) with respect to the susceptibility of the upper respiratory tract mucosa to Haemophilus influenzae. Human nasal polyps in outgrowth culture were used to study H influenzae disturbance of the ciliary beat frequency (CBF) and the morphology of cilia. The CBF of control primary culture was 11.7 +/- 2.7 Hz. The CBF slowed down significantly, to 8.5 +/- 5.7 Hz, after incubation with the filtrate of 10(8) CFU/mL of H influenzae, and to 4.1 +/- 4.1 Hz with a suspension of 10(8) CFU/mL of H influenzae (p<.05). In the morphologic study, we classified the shapes of the cilia into five types: normal cilia, immotile cilia, swollen cilia, clumped cilia, and exfoliated ciliated cells. The abnormal shapes of cilia increased after incubation with the suspension or the filtrate of H influenzae. By scanning electron microscopy, we saw that aggregated bacteria tended to associate with cilia. Thus, the findings suggest that H influenzae disturbs the ciliary clearance of nasal cells and makes them more susceptible to infections.
我们聚焦于分泌性中耳炎（OME）的致病性，涉及上呼吸道黏膜对流感嗜血杆菌的易感性。采用体外培养的人鼻息肉来研究流感嗜血杆菌对纤毛摆动频率（CBF）及纤毛形态的影响。对照原代培养的CBF为11.7±2.7Hz。与10⁸CFU/mL流感嗜血杆菌的滤液孵育后，CBF显著减慢至8.5±5.7Hz，与10⁸CFU/mL流感嗜血杆菌的悬液孵育后则减慢至4.1±4.1Hz（p<0.05）。在形态学研究中，我们将纤毛形状分为五种类型：正常纤毛、不动纤毛、肿胀纤毛、成簇纤毛和脱落的纤毛细胞。与流感嗜血杆菌悬液或滤液孵育后，异常纤毛形状增加。通过扫描电子显微镜观察，我们发现聚集的细菌倾向于与纤毛结合。因此，这些发现表明流感嗜血杆菌会干扰鼻细胞的纤毛清除功能，使其更易受到感染。
