Morikawa Y, Ishihara Y, Tohya K, Kakudo K, Seo M K, Matsuura N
Department of Pathology, Wakayama Medical School, Japan.
Arch Pathol Lab Med. 1996 May;120(5):490-6.
To characterize the distribution of the receptor for the fibroblast growth factor in human normal tissues and tumors using a new monoclonal antibody.
Monoclonal antibodies for a kinase-insert region of fibroblast growth factor receptor-1 (FGFR-1) were generated. We conducted an immunohistological analysis of FGFR-1 using a highly specific antibody we generated, and we examined the distribution of this receptor in normal human tissues and in tumors.
Intense positivity of FGFR-1 was observed in astrocytes in the brain, smooth muscles in the uterus, cardiac myocytes, respiratory epithelium in the lung, tubular epithelium in the kidney, acinar cells in the pancreas, follicular cells in the thyroid, and ductal and lobular epithelium in the breast. We also observed FGFR-1 expression in the fibroblasts and the tissue microvasculature. In addition to some nonepithelial tumors, some epithelial tumors expressed FGFR-1, including pancreatic adenocarcinomas, thyroid papillary carcinomas, invasive ductal carcinomas of the breast, lung adenocarcinomas, renal cell carcinomas, and colonic adenocarcinomas. Although FGFR-1 was expressed in colonic adenocarcinomas, which have invasive potential, tubular adenomas, which are noninvasive, did not express FGFR-1.
We have been able to define the distribution of FGFR-1 in human normal tissues and tumors. Especially in colonic tumors, FGFR-1 expression may lead adenoma cells to invade and grow in the surrounding tissue.
使用一种新的单克隆抗体来描述成纤维细胞生长因子受体在人正常组织和肿瘤中的分布情况。
制备针对成纤维细胞生长因子受体-1（FGFR-1）激酶插入区的单克隆抗体。我们使用自行制备的高特异性抗体对FGFR-1进行免疫组织学分析，并检测该受体在人正常组织和肿瘤中的分布。
在脑内星形胶质细胞、子宫平滑肌、心肌细胞、肺呼吸上皮、肾肾小管上皮、胰腺腺泡细胞、甲状腺滤泡细胞以及乳腺导管和小叶上皮中观察到FGFR-1呈强阳性。我们还在成纤维细胞和组织微血管中观察到FGFR-1表达。除了一些非上皮性肿瘤外，一些上皮性肿瘤也表达FGFR-1，包括胰腺腺癌、甲状腺乳头状癌、乳腺浸润性导管癌、肺腺癌、肾细胞癌和结肠腺癌。虽然FGFR-1在具有侵袭潜能的结肠腺癌中表达，但无侵袭性的管状腺瘤不表达FGFR-1。
我们已能够明确FGFR-1在人正常组织和肿瘤中的分布。特别是在结肠肿瘤中，FGFR-1表达可能导致腺癌细胞侵袭并在周围组织中生长。
