de Wilde P C, Kater L, Bodeutsch C, van den Hoogen F H, van de Putte L B, van Venrooij W J
University Hospital, Nijmegen, The Netherlands.
Arthritis Rheum. 1996 May;39(5):783-91. doi: 10.1002/art.1780390510.
Salivary glands of patients with Sjögren's syndrome (SS) have been shown to be a site of anti-SS-B/La antibody production. The present study investigated differences in the localization of the SS-B/La antigen in labial salivary gland (LSG) tissue between SS and non-SS patients, which may explain the local antigen-driven anti-SS-B/La response.
Distribution of SS-B/La was studied immunohistologically in the LSG biopsy samples of 9 SS patients, 10 non-SS patients, and in normal tissues obtained at autopsy within 2 hours after death, using a mouse monoclonal antibody directed to SS-B/La. In 3 SS and 3 non-SS patients, LSGs were also studied with affinity-purified biotinylated human antibodies directed against SS-B/La.
In the non-SS patients, SS-B/La was primarily observed in the nucleoli of acinic cells of the LSGs. Patients with either primary SS or secondary SS showed an accumulation of SS-B/La in the nucleoplasm of acinic cells. In the SS patients, SS-B/La was also detected in the cytoplasm as a diffuse or perinuclear staining. Sometimes, SS-B/La was found along the membrane of acinic cells as well. This aberrant nuclear and cytoplasmic distribution of SS-B/La in SS patients correlated well with abnormalities in the composition of the plasma cell population in the LSGs, but not with a lymphocytic focus score > 1.
The accumulation and redistribution of SS-B/La in the LSGs may play an important role in the local antigen-driven anti-SS-B/La response in SS, and can also be used to improve the diagnostic possibilities of the LSG biopsy.
已有研究表明，干燥综合征（SS）患者的唾液腺是抗SS - B/La抗体的产生部位。本研究调查了SS患者与非SS患者唇腺（LSG）组织中SS - B/La抗原定位的差异，这可能解释局部抗原驱动的抗SS - B/La反应。
使用针对SS - B/La的小鼠单克隆抗体，对9例SS患者、10例非SS患者的LSG活检样本以及死后2小时内尸检获得的正常组织进行免疫组织学研究，观察SS - B/La的分布情况。对3例SS患者和3例非SS患者的LSG，还使用亲和纯化的生物素化抗SS - B/La人抗体进行了研究。
在非SS患者中，SS - B/La主要见于LSG腺泡细胞的核仁。原发性SS或继发性SS患者的腺泡细胞核质中可见SS - B/La聚集。在SS患者中，还可在细胞质中检测到弥漫性或核周染色的SS - B/La。有时，在腺泡细胞膜上也能发现SS - B/La。SS患者中SS - B/La这种异常的核质分布与LSG中浆细胞群体组成的异常密切相关，但与淋巴细胞灶性评分>1无关。
LSG中SS - B/La的聚集和重新分布可能在SS局部抗原驱动的抗SS - B/La反应中起重要作用，也可用于提高LSG活检的诊断准确性。
