Hudson J A, Mackenzie C A, Joblin K N
AgResearch, Grasslands Research Centre, Palmerston North, New Zealand.
Appl Microbiol Biotechnol. 1996 Apr;45(3):404-7. doi: 10.1007/s002530050703.
A ruminal strain of Enterococcus faecalis was characterised with respect to its ability to hydrate oleic acid to 10-hydroxystearic acid. Hydroxy fatty acid was produced after growth had ceased and the carbon source was almost exhausted. Hydroxy fatty acid production was equally rapid whether the inoculum had been grown in the presence of oleic acid or not, and almost complete conversion was achieved when oleic acid was present at a concentration of up to 0.5% (v/v). Incubation under a hydrogen headspace did not result in biohydrogenation of oleic acid. In pH-controlled batch culture the proportion of oleic acid hydrated varied with the pH of incubation, with more hydration at lower pH. Growth was retarded in the presence of 0.1% (v/v) linoleic acid, inhibited by the same concentration of linolenic acid and did not result in the formation of hydrated products from these substrates. If this organism is able to transform oleic acid in the rumen then the only product likely to be formed is 10-hydroxystearic acid.
对一株粪肠球菌的瘤胃菌株进行了表征，研究其将油酸水合生成10-羟基硬脂酸的能力。在生长停止且碳源几乎耗尽后产生了羟基脂肪酸。无论接种物是否在油酸存在的情况下生长，羟基脂肪酸的产生速度相同，当油酸浓度高达0.5%（v/v）时，几乎可实现完全转化。在氢气顶空条件下培养不会导致油酸生物氢化。在pH控制的分批培养中，水合油酸的比例随培养pH值而变化，在较低pH值下有更多的水合作用。在0.1%（v/v）亚油酸存在下生长受到抑制，相同浓度的亚麻酸会抑制生长，且不会从这些底物形成水合产物。如果这种微生物能够在瘤胃中转化油酸，那么可能形成的唯一产物就是10-羟基硬脂酸。
