Bevers Loes E, Pinkse Martijn W H, Verhaert Peter D E M, Hagen Wilfred R
Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
J Bacteriol. 2009 Aug;191(15):5010-2. doi: 10.1128/JB.00306-09. Epub 2009 May 22.
The hydration of oleic acid into 10-hydroxystearic acid was originally described for a Pseudomonas cell extract almost half a century ago. In the intervening years, the enzyme has never been characterized in any detail. We report here the isolation and characterization of oleate hydratase (EC 4.2.1.53) from Elizabethkingia meningoseptica.
近半个世纪前,人们首次在假单胞菌细胞提取物中发现油酸水合生成10-羟基硬脂酸的反应。在此后的数年里,这种酶从未得到过详细的表征。我们在此报告了从脑膜伊丽莎白菌中分离并鉴定油酸水合酶(EC 4.2.1.53)的过程。