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在糖基化抑制剂存在的情况下产生的人干扰素分子种类的改变。

Altered molecular species of human interferon produced in the presence of inhibitors of glycosylation.

作者信息

Havell E A, Yamazaki S, Vilcek J

出版信息

J Biol Chem. 1977 Jun 25;252(12):4425-7.

PMID:863934
Abstract

The inhibitors of glycosylation, 2-deoxy-D-glucose or D-glucosamine, inhibit the synthesis of biologically active interferon in human FS-4 fibroblast cultures stimulated with polyinosinate-polycytidylate. Interferon synthesized in the presence of partially inhibitory concentrations of 2-deoxy-D-glucose or D-glucosamine were found to differ from interferons made in control cultures in some physical properties. Interferons synthesized in the presence of either inhibitor had a diminished charge heterogeneity demonstrable by iso-electric focusing. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, control interferon activity formed a single peak with the apparent molecular weight of 20,000, whereas interferons from cultures treated with either inhibitor could be resolved into two distinct molecular weight components, one of which was smaller than the interferon synthesized in control cultures.

摘要

糖基化抑制剂2-脱氧-D-葡萄糖或D-氨基葡萄糖，可抑制在聚肌苷酸-聚胞苷酸刺激的人FS-4成纤维细胞培养物中生物活性干扰素的合成。发现在部分抑制浓度的2-脱氧-D-葡萄糖或D-氨基葡萄糖存在下合成的干扰素，在某些物理性质上与对照培养物中产生的干扰素不同。在任何一种抑制剂存在下合成的干扰素，通过等电聚焦可显示电荷异质性降低。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中，对照干扰素活性形成一个单一峰，表观分子量为20,000，而用任何一种抑制剂处理的培养物中的干扰素可分解为两个不同的分子量组分，其中一个比对照培养物中合成的干扰素小。

相似文献

1
Altered molecular species of human interferon produced in the presence of inhibitors of glycosylation.在糖基化抑制剂存在的情况下产生的人干扰素分子种类的改变。
J Biol Chem. 1977 Jun 25;252(12):4425-7.
2
The effect of inhibitors of glycosylation on interferon production in human lymphoblastoid cells.糖基化抑制剂对人淋巴母细胞样细胞中干扰素产生的影响。
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Effect of glycosylation inhibitors on the production and properties of human leukocyte interferon.糖基化抑制剂对人白细胞干扰素产生及特性的影响
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Suppression of human interferon production by inhibitors of glycosylation.糖基化抑制剂对人干扰素产生的抑制作用。
Virology. 1975 Feb;63(2):475-83. doi: 10.1016/0042-6822(75)90320-7.
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Interferoids: in vitro and in vivo conversion of native interferons to lower molecular weight forms.干扰素诱导物：天然干扰素在体外和体内向低分子量形式的转化。
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引用本文的文献

1
The glycoprotein nature of murine gamma interferon.小鼠γ干扰素的糖蛋白性质
Arch Virol. 1984;80(2-3):195-207. doi: 10.1007/BF01310659.
2
Constitutive, long-term production of human interferons by hamster cells containing multiple copies of a cloned interferon gene.含有克隆干扰素基因多个拷贝的仓鼠细胞持续长期产生人干扰素。
Nucleic Acids Res. 1983 Feb 11;11(3):687-706. doi: 10.1093/nar/11.3.687.
3
Structure and expression of cloned murine IFN-alpha genes.克隆的小鼠α干扰素基因的结构与表达
Nucleic Acids Res. 1983 Feb 11;11(3):555-73. doi: 10.1093/nar/11.3.555.
4
Enhanced production of murine interferon gamma by T cells generated in response to bacterial infection.因细菌感染而产生的T细胞增强了小鼠γ干扰素的生成。
J Exp Med. 1982 Jul 1;156(1):112-27. doi: 10.1084/jem.156.1.112.
5
Differences between human alpha (leukocyte) and beta (fibroblast) interferons.人α（白细胞）干扰素和β（成纤维细胞）干扰素之间的差异。
Arch Virol. 1981;67(4):267-81. doi: 10.1007/BF01314831.
6
Evidence for a unique human fibroblast interferon (IFN-beta 1) chromosomal gene, devoid of intervening sequences.关于一种独特的人类成纤维细胞干扰素（IFN-β1）染色体基因的证据，该基因无间隔序列。
Nucleic Acids Res. 1981 Feb 11;9(3):461-71. doi: 10.1093/nar/9.3.461.
7
Enzymatic modifications of human fibroblast and leukocyte interferons.人成纤维细胞干扰素和白细胞干扰素的酶促修饰
J Virol. 1980 Aug;35(2):390-9. doi: 10.1128/JVI.35.2.390-399.1980.
8
The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.使用逆转录酶的合成寡聚脱氧核糖核苷酸引物推导的人成纤维细胞干扰素的完整氨基酸序列。
Nucleic Acids Res. 1980 Jul 11;8(13):2885-94. doi: 10.1093/nar/8.13.2885.
9
Glycosylation of human leukocyte interferon: effects of tunicamycin.人白细胞干扰素的糖基化：衣霉素的作用
Arch Virol. 1980;64(2):109-17. doi: 10.1007/BF01318014.
10
Characterization of a mouse interferon gene locus I. Isolation of a cluster of four alpha interferon genes.小鼠干扰素基因位点的特征分析I. 四个α干扰素基因簇的分离
Nucleic Acids Res. 1985 Feb 11;13(3):805-23. doi: 10.1093/nar/13.3.805.