关于一种独特的人类成纤维细胞干扰素（IFN-β1）染色体基因的证据，该基因无间隔序列。
Evidence for a unique human fibroblast interferon (IFN-beta 1) chromosomal gene, devoid of intervening sequences.
作者信息
Tavernier J, Derynck R, Fiers W
出版信息
Nucleic Acids Res. 1981 Feb 11;9(3):461-71. doi: 10.1093/nar/9.3.461.
Direct restriction analysis of the human genome, using the Southern transfer technique and hybridization with a human fibroblast interferon (IFN-beta) complementary DNA insert probe, revealed the presence of a single gene; no additional closely related IFN-beta genes could be detected. A lambda-linked human gene library (Lawn et al., Cell, 15, 1157-1174 (1978)) was screened using the cDNA probe. Out of 600,000 recombinant phage examined, one single clone bearing interferon sequences was obtained. Restriction analysis of the relevant region revealed an identical restriction map as obtained for the IFN-beta 1 cDNA clones. No intervening sequences could be detected, either in the coding or the non-coding regions of the gene.
利用Southern转移技术并与人成纤维细胞干扰素（IFN-β）互补DNA插入探针杂交，对人类基因组进行直接限制性分析，结果显示存在单个基因；未检测到其他密切相关的IFN-β基因。使用该cDNA探针筛选了一个λ噬菌体连接的人类基因文库（Lawn等人，《细胞》，15卷，1157 - 1174页（1978年））。在检查的600,000个重组噬菌体中，获得了一个携带干扰素序列的单个克隆。对相关区域的限制性分析显示，其限制性图谱与IFN-β1 cDNA克隆的图谱相同。在该基因的编码区或非编码区均未检测到间隔序列。
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