Houghton M, Easton M A, Stewart A G, Smith J C, Doel S M, Catlin G H, Lewis H M, Patel T P, Emtage J S, Carey N H, Porter A G
Nucleic Acids Res. 1980 Jul 11;8(13):2885-94. doi: 10.1093/nar/8.13.2885.
Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous with the mRNA sequence coding for amino acids 42-45 and used it as a primer to selectively transcribe an interferon cDNA template. The sequence of the newly synthesised DNA predicted the sequence of amino acids 48-109 in the interferon polypeptide. By repeating this process with one more primer, we have determined the complete amino acid sequence of mature human fibroblast interferon, a polypeptide of 166 amino acids.
我们最近通过使用合成的寡聚脱氧核糖核苷酸引发干扰素mRNA和cDNA的转录，确定了编码成熟人成纤维细胞干扰素47个氨基末端氨基酸的mRNA序列(1)。根据这个序列，我们现在合成了一种与编码氨基酸42 - 45的mRNA序列同源的寡聚脱氧核糖核苷酸，并将其用作引物来选择性转录干扰素cDNA模板。新合成的DNA序列预测了干扰素多肽中氨基酸48 - 109的序列。通过再用一种引物重复这个过程，我们确定了成熟人成纤维细胞干扰素（一种由166个氨基酸组成的多肽）的完整氨基酸序列。
