Knowles R W, Person S
J Virol. 1976 May;18(2):644-51. doi: 10.1128/JVI.18.2.644-651.1976.
2-Deoxyglucose and glucosamine were found to inhibit cell fusion caused by a syncytial mutant of herpes simplex virus and to inhibit the glycosylation of viral glycoproteins in the infected cells. The inhibition of fusion and the inhibition of glycosylation caused by 2-deoxyglucose were substantially prevented when mannose was also present during infection. When glycosylation was inhibited, three new bands were found in major glycoprotein region on sodium dodecyl sulfate-polyacrylamide gels. These bands may be precursors to the normal glycoproteins. The correlation between fusion and glycosylation in the presence of 2-deoxyglucose, glucosamine, and mannose suggests that the cells cannot fuse if their glycoproteins have a considerably reduced carbohydrate content.
已发现2-脱氧葡萄糖和氨基葡萄糖可抑制单纯疱疹病毒合胞体突变体引起的细胞融合，并抑制受感染细胞中病毒糖蛋白的糖基化。当感染期间同时存在甘露糖时，2-脱氧葡萄糖对融合的抑制作用以及对糖基化的抑制作用可得到显著缓解。当糖基化受到抑制时，在十二烷基硫酸钠-聚丙烯酰胺凝胶的主要糖蛋白区域发现了三条新条带。这些条带可能是正常糖蛋白的前体。2-脱氧葡萄糖、氨基葡萄糖和甘露糖存在时融合与糖基化之间的相关性表明，如果细胞的糖蛋白碳水化合物含量大幅降低，细胞就无法融合。
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