Kang K Y, Bice D, Hoffman E, D'Amato R, Salvaggio J
J Allergy Clin Immunol. 1977 Jun;59(6):425-36. doi: 10.1016/0091-6749(77)90005-7.
In a study designed to assess the potential sensitizing and granulomagenic capacities of selected metallic salts, rabbits were inoculated intradermally with zirconium aluminum glycinate (ZAG), sodium zirconium lactate (NZL), aluminum chlorhydrate (ACH), BeSO 4, and ovalbumin (OVA) by single and multiple injections. Animals immunized with BeSO4 and with OVA developed delayed skin reactivity as well as antigen-specific alveolar macrophage migration inhibition. Neither single nor multiple injections of ZAG or ACH resulted in clear-cut positive skin reactivity, macrophage migration inhibitory factor (MIF) production, or lymphocyte stimulation. Rabbits inoculated with multiple injections of NZL (500 microng) showed some marginally positive macrophage migration inhibition and skin reactivity. Histologically, ZAG and ACH were found to induce well-organized foreign-body granulomas after intradermal injection in both normal and inoculated rabbits. NZL and BeSO4 also induced skin granulomas, but these were less organized and distinct. Cell viability and ultrastructural studies indicated that BeSO4 was highly toxic for isolated alveolar macrophages in vitro at concentrations above 10 microng/ml, but NZL and ZAG did not exert such an effect at these dose levels. BeSO4 also depressed lymphocyte stimulation in sensitized animals which demonstrated delayed skin reactivity and macrophage migration inhibition.
在一项旨在评估所选金属盐潜在致敏和致肉芽肿能力的研究中，通过单次和多次注射，给兔子皮内接种甘氨酸锆铝（ZAG）、乳酸锆钠（NZL）、碱式氯化铝（ACH）、硫酸铍（BeSO₄）和卵清蛋白（OVA）。用BeSO₄和OVA免疫的动物出现了延迟性皮肤反应以及抗原特异性肺泡巨噬细胞迁移抑制。单次或多次注射ZAG或ACH均未导致明确的阳性皮肤反应、巨噬细胞迁移抑制因子（MIF）产生或淋巴细胞刺激。多次注射NZL（500微克）的兔子表现出一些轻微阳性的巨噬细胞迁移抑制和皮肤反应。组织学上，在正常和接种的兔子皮内注射后，发现ZAG和ACH可诱导组织良好的异物肉芽肿。NZL和BeSO₄也诱导了皮肤肉芽肿，但这些肉芽肿组织性较差且不明显。细胞活力和超微结构研究表明，浓度高于10微克/毫升时，BeSO₄在体外对分离的肺泡巨噬细胞具有高毒性，但在这些剂量水平下，NZL和ZAG没有这种作用。BeSO₄还抑制了致敏动物的淋巴细胞刺激，这些动物表现出延迟性皮肤反应和巨噬细胞迁移抑制。
