Bizbiz L, Bonithon-Kopp C, Ducimetierè P, Berr C, Alperovitch A, Robert L
Laboratoire de Biologie Cellulaire, Paris, France.
Atherosclerosis. 1996 Feb;120(1-2):47-55. doi: 10.1016/0021-9150(95)05676-9.
The potential interest of serum elastase activity (SEA) as a marker of vascular aging and atherosclerosis was studied as part of an epidemiological study on vascular and cognitive aging (EVA Study). SEA was measured in 555 men and 774 women aged 59-71 years with a synthetic substrate, suc(ala)3pNA, according to a modified enzyme-linked immunosorbant assay (ELISA)-type procedure. The distribution of SEA-values was skewed to the right in men and women, the mean value was 0.52 +/- 0.55 U/ml in males and 0.43 +/- 0.52 U/ml for females. This difference could be entirely explained by alcohol consumption. SEA increased strongly with alcohol consumption in males and females. It was also positively and significantly correlated with body mass index (BMI) and systolic blood pressure (SBP). SEA significantly decreased with age in men and was not influenced by smoking in either sex. SEA was significantly increased in diabetic men compared with non-diabetics and a similar trend, although not significant, was observed in women. When both sexes were combined, the association between diabetes and SEA was independent of other clinical risk factors. No significant associations were observed with intima-media thickness or atherosclerotic plaques assessed by B-mode carotid ultrasonography. Among biological risk factors, triglycerides (in both sexes) and glucose (in men) appeared the strongest correlates of increase in SEA. In multivariate analysis, independent determinants of an increased SEA were age, alcohol consumption, triglycerides and glucose in men, and alcohol consumption and triglycerides in women.
作为一项关于血管和认知衰老的流行病学研究（EVA研究）的一部分，对血清弹性蛋白酶活性（SEA）作为血管衰老和动脉粥样硬化标志物的潜在价值进行了研究。采用改良的酶联免疫吸附测定（ELISA）法，使用合成底物suc(ala)3pNA对555名年龄在59 - 71岁的男性和774名女性进行SEA测量。男性和女性的SEA值分布均向右偏态，男性的平均值为0.52±0.55 U/ml，女性为0.43±0.52 U/ml。这种差异完全可以由饮酒来解释。男性和女性的SEA均随饮酒量显著增加。它还与体重指数（BMI）和收缩压（SBP）呈正相关且具有显著性。男性的SEA随年龄显著降低，且在两性中均不受吸烟影响。与非糖尿病男性相比，糖尿病男性的SEA显著升高，女性也观察到类似趋势，尽管不显著。当两性合并时，糖尿病与SEA之间的关联独立于其他临床风险因素。通过B型颈动脉超声评估的内膜中层厚度或动脉粥样硬化斑块未观察到显著关联。在生物学风险因素中，甘油三酯（在两性中）和葡萄糖（在男性中）似乎是SEA升高的最强相关因素。在多变量分析中，男性SEA升高的独立决定因素是年龄、饮酒、甘油三酯和葡萄糖，女性是饮酒和甘油三酯。
