Gjøoen K, Olsen A O, Magnus P, Grinde B, Sauer T, Orstavik I
Department of Virology, National Institute of Public Health, Oslo, Norway.
APMIS. 1996 Jan;104(1):68-74. doi: 10.1111/j.1699-0463.1996.tb00688.x.
HPV is suspected of being a major cause of cancer of the uterine cervix. To understand the risk of disease in the general population of women, it is important to estimate the prevalence of HPV infection in a random population-based sample of women without disease. In this study, a total of 231 randomly selected women without dysplasia (controls) were examined, and compared with 103 women with histologically confirmed CIN II-III (patients). The prevalence of HPV DNA in cervical scrapes was determined by general nested PCR, which was expected to detect any relevant HPV type commonly found in cervical samples. The nested positive samples were typed with type-specific PCR. In the general nested PCR, 15% of the controls were positive, compared to 91% of the patients. In the population-based sample, 2.2% had HPV types 6 and 11 and 10% had types 16, 18, 31, and 33. In both groups, HPV DNA was observed less frequently in women above than below the age of 30. The results are among the few population-based figures on the prevalence of HPV in women, and provide a baseline for understanding the risk of developing cancer of the uterine cervix, and determining the proportion of women to be included in intervention studies.
人乳头瘤病毒（HPV）被怀疑是子宫颈癌的主要病因。为了解普通女性人群的患病风险，在基于人群随机抽样的无病女性样本中估计HPV感染率很重要。在本研究中，共检查了231名随机选取的无发育异常的女性（对照组），并与103名经组织学确诊为CIN II - III级的女性（患者组）进行比较。通过通用巢式PCR检测宫颈刮片中HPV DNA的感染率，该方法有望检测出宫颈样本中常见的任何相关HPV类型。巢式PCR阳性样本用型特异性PCR进行分型。在通用巢式PCR中，对照组15%呈阳性，而患者组为91%。在基于人群的样本中，2.2%的人感染了HPV 6型和11型，10%的人感染了16、18、31和33型。在两组中，30岁以上女性中HPV DNA的检出率低于30岁以下女性。这些结果是为数不多的基于人群的女性HPV感染率数据之一，为了解子宫颈癌发病风险以及确定纳入干预研究的女性比例提供了基线。
