Granerus M, Schofield P, Bierke P, Engström W
Department of Pathology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Int J Dev Biol. 1995 Oct;39(5):759-64.
The balance between different cell populations in the developing organism is controlled by regulating the rates of multiplication, differentiation or death of its constituent cells. The human teratocarcinoma derived cell line Tera 2, which in several aspects mirrors early embryonic cells, can be induced to undergo programmed cell death (apoptosis) by depriving cell cultures of serum. This study demonstrates that this process can be reversed by replacing serum with physiological concentrations of insulin like growth factor I (IGF I). As a result, IGF I enhances the rate of Tera 2 cell proliferation in serum free medium. In contrast, Transforming Growth Factor beta1 did not exert any effect on growth or apoptosis in Tera 2 cells. The results indicate that one effect of growth factors on pluripotential cells is to regulate the balance between cell proliferation and cell death.
发育中的生物体中不同细胞群体之间的平衡是通过调节其组成细胞的增殖、分化或死亡速率来控制的。源自人畸胎癌的细胞系Tera 2在几个方面反映了早期胚胎细胞，通过剥夺细胞培养液中的血清，可诱导其发生程序性细胞死亡（凋亡）。本研究表明，用生理浓度的胰岛素样生长因子I（IGF I）替代血清可逆转这一过程。结果，IGF I提高了Tera 2细胞在无血清培养基中的增殖速率。相比之下，转化生长因子β1对Tera 2细胞的生长或凋亡没有任何影响。结果表明，生长因子对多能细胞的一个作用是调节细胞增殖与细胞死亡之间的平衡。
