Mohabir L A, Rees T J
National Blood Transfusion Service (Wales), Rhydlafar, Nr St Fagans, Cardiff, UK.
Transfus Med. 1995 Dec;5(4):275-9. doi: 10.1111/j.1365-3148.1995.tb00214.x.
Severe or life-threatening anaphylactic reactions during blood transfusion to patients with anti-IgA, though infrequent, can be avoided by transfusing blood products prepared from IgA deficient donors. This report describes the development and use a passive haemagglutination assay adapted for use on the Olympus PK7100 blood group analyser. Donor plasma samples identified as being presumptive IgA deficient were further screened by a manual passive haemagglutination inhibition technique and finally confirmed by means of a quantitative enzyme-linked immunosorbent assay. Of 5723 donors tested simultaneously for IgA deficiency, blood grouping and red cell antibody screening, six (1 in 954) were confirmed as IgA deficient (i.e. < 100 ng/ml of IgA). The test is simple and cost effective and can be used to establish a database of IgA-deficient donors.
给抗IgA患者输血时发生的严重或危及生命的过敏反应虽然不常见，但通过输注由IgA缺乏供体制备的血液制品可以避免。本报告描述了一种适用于奥林巴斯PK7100血型分析仪的被动血凝试验的开发和应用。通过手工被动血凝抑制技术对被鉴定为推定IgA缺乏的供体血浆样本进行进一步筛选，最终通过定量酶联免疫吸附试验进行确认。在同时检测IgA缺乏、血型和红细胞抗体筛查的5723名供体中，有6名（954名中有1名）被确认为IgA缺乏（即IgA<100 ng/ml）。该检测方法简单且成本效益高，可用于建立IgA缺乏供体数据库。
