Marcellino L H, Neshich G, Grossi de Sá M F, Krebbers E, Gander E S
EMBRAPA-CENARGEN S.A.I.N., Brasília DF, Brazil.
FEBS Lett. 1996 May 6;385(3):154-8. doi: 10.1016/0014-5793(96)00375-4.
Brazil nut 2S albumins lack the essential amino acid tryptophan. In order to improve the protein's nutritional value and create a basis for structural investigations, three separate modified Brazil nut 2S albumin genes were constructed. The first mutant contains five consecutive tryptophan codons, while the other two modified genes encode proteins carrying single tryptophan residues at sites that will allow confirmation of the predicted protein structure through fluorescence quenching techniques. The modified genes, under the regulation of the CaMV 35S promoter, were introduced into Nicotiana tabacum. All three modified genes were correctly transcribed and the 2S albumin accumulated in the seeds of transgenic plants.
巴西坚果2S白蛋白缺乏必需氨基酸色氨酸。为了提高该蛋白质的营养价值并为结构研究奠定基础，构建了三个独立的修饰巴西坚果2S白蛋白基因。第一个突变体包含五个连续的色氨酸密码子，而另外两个修饰基因编码的蛋白质在特定位点携带单个色氨酸残基，这将通过荧光猝灭技术来确认预测的蛋白质结构。在花椰菜花叶病毒35S启动子的调控下，将修饰基因导入烟草。所有三个修饰基因均正确转录，并且2S白蛋白在转基因植物种子中积累。
