Takahashi S, Oida K, Ookubo M, Suzuki J, Kohno M, Murase T, Yamamoto T, Nakai T
Third Department of Internal Medicine, Fukui Medical School, Japan.
FEBS Lett. 1996 May 20;386(2-3):197-200. doi: 10.1016/0014-5793(96)00439-5.
The VLDL receptor, a newly identified lipoprotein receptor, recognizes apoE containing lipoproteins. The human VLDL receptor was overexpressed in 1d1A-7, a mutant Chinese hamster ovary cells lacking LDL receptors. Each VLDL obtained from a normolipidemic subject with two epsilon3 or epsilon2 alleles similarly competed for the binding of radiolabeled rabbit beta-VLDL to the VLDL receptors. The anti-apoE monoclonal antibody 1D7, which inhibited binding of apoE3 to the LDL receptors, failed to compete for the binding of VLDL (apoE3 or apoE2) to the VLDL receptors. Results indicate that the binding site of apoE on the VLDL receptor may differ from its binding site on the LDL receptor.
极低密度脂蛋白（VLDL）受体是一种新发现的脂蛋白受体，可识别含载脂蛋白E（apoE）的脂蛋白。人VLDL受体在1d1A - 7细胞中过表达，1d1A - 7是一种缺乏低密度脂蛋白（LDL）受体的中国仓鼠卵巢突变细胞。从具有两个ε3或ε2等位基因的血脂正常受试者获得的每种VLDL，同样能竞争放射性标记的兔β - VLDL与VLDL受体的结合。抗apoE单克隆抗体1D7可抑制apoE3与LDL受体的结合，但不能竞争VLDL（apoE3或apoE2）与VLDL受体的结合。结果表明，apoE在VLDL受体上的结合位点可能与其在LDL受体上的结合位点不同。
