Nelson J C, Sorrells M E, Van Deynze A E, Lu Y H, Atkinson M, Bernard M, Leroy P, Faris J D, Anderson J A
Department of Plant Breeding and Biometry, Cornell University, Ithaca, New York 14853, USA.
Genetics. 1995 Oct;141(2):721-31. doi: 10.1093/genetics/141.2.721.
A molecular-marker linkage map of hexaploid wheat (Triticum aestivum L. em. Thell) provides a framework for integration with teh classical genetic map and a record of the chromosomal rearrangements involved in the evolution of this crop species. We have constructed restriction fragment length polymorphism (RFLP) maps of the A-, B-, and D-genome chromosomes of homoeologous groups 4, 5, and 7 of wheat using 114 F7 lines from a synthetic X cultivated wheat cross and clones from 10 DNA libraries. Chromosomal breakpoints for known ancestral reciprocal translocations involving these chromosomes and for a known pericentric inversion on chromosome 4A were localized by linkage and aneuploid analysis. Known genes mapped include the major vernalization genes Vrn1 and Vrn3 on chromosome arms 5AL and 5DL, the red-coleoptile gene Rc1 on 7AS, and presumptively the leaf-rust (Puccinia recondita f.sp. tritici) resistance gene Lr34 on 7DS and the kernel-hardness gene Ha on 5DS. RFLP markers previously obtained for powdery-mildew (Blumeria graminis f.sp. tritici) resistance genes Pm2 and Pm1 were localized on chromosome arms 5DS and 7AL.
六倍体小麦（Triticum aestivum L. em. Thell）的分子标记连锁图谱为与经典遗传图谱整合提供了框架，并记录了该作物物种进化过程中涉及的染色体重排。我们利用一个人工合成小麦与栽培小麦杂交产生的114个F7株系以及来自10个DNA文库的克隆，构建了小麦同源群4、5和7的A、B、D基因组染色体的限制性片段长度多态性（RFLP）图谱。通过连锁分析和非整倍体分析，定位了涉及这些染色体的已知祖先相互易位的染色体断点以及4A染色体上一个已知的臂内倒位。已定位的已知基因包括位于5AL和5DL染色体臂上的主要春化基因Vrn1和Vrn3、位于7AS上的红胚芽鞘基因Rc1，以及推测位于7DS上的叶锈病（Puccinia recondita f.sp. tritici）抗性基因Lr34和位于5DS上的籽粒硬度基因Ha。先前获得的白粉病（Blumeria graminis f.sp. tritici）抗性基因Pm2和Pm1的RFLP标记定位在5DS和7AL染色体臂上。
