Geng Hongwei, Shi Jia, Fuerst E Patrick, Wei Jingxin, Morris Craig F
College of Agronomy, Xinjiang Agricultural University, Ürümqi, China.
Department of Crop and Soil Sciences, Washington State University, Pullman, WA, United States, Affiliated with the Western Wheat Quality Laboratory.
Front Plant Sci. 2019 Apr 24;10:523. doi: 10.3389/fpls.2019.00523. eCollection 2019.
Peroxidase (POD) activity in wheat ( L.) grain influences natural carotenoid pigment content and is associated with the color of flour, and processing and product quality. Here, we report the molecular characterization and physical mapping of POD genes in bread wheat. The complete genomic DNA (gDNA) sequence of two POD genes ( and ), and the partial gDNA sequence of two additional POD genes ( and ) from wheat were characterized using cloning and validated through laboratory experiments. Using a set of 21 nullisomic-tetrasomic (NT) lines, six group-7 ditelosomic (Dt) lines, and 38 group-7 deletion (Del) lines of Chinese Spring (CS), and were found to be physically located on 0.73-0.83 and on the most distal 0.39 fraction arm length (FL) of 7AS and 7DS in cv. CS, respectively; whereas, and were assigned to the 0.40-0.49 and 0.40-0.48 FL of 7AL and 7BL, respectively. Based on single nucleotide polymorphisms (SNPs) of two alleles at the locus, two functional markers POD-7D1 and POD-7D6 were developed, amplifying 540- and 640-bp, fragments in varieties with higher and lower POD activities, respectively. A total of 224 wheat varieties were analyzed and showed a significant association between the polymorphic fragments and POD activity using POD-7D1 and POD-7D6 markers. The analysis of variance (ANOVA) indicated the average POD activities of 115 varieties with - were significantly lower than 109 varieties with - ( < 0.01). This study provides useful information of the POD genes in bread wheat, insight into wheat genome synteny and structure, gene-specific markers, and contributes a valuable resource for quality improvement in wheat breeding programs.
小麦籽粒中的过氧化物酶（POD）活性影响天然类胡萝卜素色素含量，并与面粉颜色、加工及产品品质相关。在此，我们报道了面包小麦中POD基因的分子特征及物理定位。利用克隆技术对小麦中两个POD基因（和）的完整基因组DNA（gDNA）序列以及另外两个POD基因（和）的部分gDNA序列进行了表征，并通过实验室实验进行了验证。利用一套中国春（CS）的21个缺体-四体（NT）系、6个7组双端体（Dt）系和38个7组缺失（Del）系，发现和分别物理定位在CS品种7AS和7DS最末端0.39的片段长度（FL）上，位于0.73 - 0.83区间；而和分别被定位到7AL和7BL的0.40 - 0.49 FL和0.40 - 0.48 FL区间。基于位点两个等位基因的单核苷酸多态性（SNP），开发了两个功能标记POD - 7D1和POD - 7D6，分别在POD活性较高和较低的品种中扩增出540 bp和640 bp的片段。共分析了224个小麦品种，使用POD - 7D1和POD - 7D6标记显示多态性片段与POD活性之间存在显著关联。方差分析（ANOVA）表明，115个具有 - 的品种的平均POD活性显著低于109个具有 - 的品种（<0.01）。本研究提供了面包小麦中POD基因的有用信息，深入了解了小麦基因组的同线性和结构、基因特异性标记，并为小麦育种计划中的品质改良贡献了宝贵资源。
