α2抑制型启动子对激活因子Gal4的可及性。
Accessibility of alpha 2-repressed promoters to the activator Gal4.
作者信息
Redd M J, Stark M R, Johnson A D
机构信息
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0414, USA.
出版信息
Mol Cell Biol. 1996 Jun;16(6):2865-9. doi: 10.1128/MCB.16.6.2865.
Abstract
It has been proposed that eukaryotic repressors of transcription can act by organizing chromatin, thereby preventing the accessibility of nearby DNA to activator proteins required for transcription initiation. In this study, we test this idea for the yeast alpha 2 repressor using a simple, artificial promoter that contains a single binding site for the activator protein Gal4 and a single binding site for the repressor alpha 2. When both the repressor and the activator are expressed in the same cell, the artificial promoter is efficiently repressed. In vivo footprinting experiments demonstrate that Gal4 can occupy its binding site even when the promoter is repressed. This result indicates that alpha 2-directed repression must result from interference with some stage in transcription initiation other than activator binding to DNA.
摘要
有人提出，真核生物转录抑制因子可通过组织染色质发挥作用，从而阻止附近DNA与转录起始所需的激活蛋白接触。在本研究中，我们使用一个简单的人工启动子来验证酵母α2抑制因子的这一观点，该启动子含有一个激活蛋白Gal4的单一结合位点和一个抑制因子α2的单一结合位点。当抑制因子和激活蛋白在同一细胞中表达时，人工启动子会被有效抑制。体内足迹实验表明，即使启动子被抑制，Gal4仍能占据其结合位点。这一结果表明，α2介导的抑制必定是由于干扰了转录起始过程中除激活蛋白与DNA结合之外的某个阶段。
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本文引用的文献
1
Transcriptional repression in eukaryotes.真核生物中的转录抑制
Annu Rev Cell Biol. 1993;9:479-509. doi: 10.1146/annurev.cb.09.110193.002403.
2
Genomic footprinting of the promoter regions of STE2 and STE3 genes in the yeast Saccharomyces cerevisiae.酿酒酵母中STE2和STE3基因启动子区域的基因组足迹分析
J Mol Biol. 1993 Dec 20;234(4):975-87. doi: 10.1006/jmbi.1993.1652.
3
Nucleosome disruption by transcription factor binding in yeast.酵母中转录因子结合导致的核小体破坏
Science. 1993 Dec 3;262(5139):1563-6. doi: 10.1126/science.8248805.
4
Chromatin analysis in yeast using NP-40 permeabilised sphaeroplasts.使用NP-40通透化的球状体进行酵母中的染色质分析。
Nucleic Acids Res. 1993 Sep 25;21(19):4653-4. doi: 10.1093/nar/21.19.4653.
5
Nucleosomal location of the STE6 TATA box and Mat alpha 2p-mediated repression.STE6基因TATA框的核小体定位及Matα2p介导的抑制作用。
Mol Cell Biol. 1994 Jun;14(6):4002-10. doi: 10.1128/mcb.14.6.4002-4010.1994.
6
Transcription: in tune with the histones.转录：与组蛋白协调一致。
Cell. 1994 Apr 8;77(1):13-6. doi: 10.1016/0092-8674(94)90229-1.
7
Positioned nucleosomes inhibit Dam methylation in vivo.定位核小体在体内抑制Dam甲基化。
Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1361-5. doi: 10.1073/pnas.91.4.1361.
8
Transcriptional repression directed by the yeast alpha 2 protein in vitro.酵母α2蛋白在体外介导的转录抑制作用
Nature. 1994 Jul 28;370(6487):309-11. doi: 10.1038/370309a0.
9
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Genes Dev. 1994 Dec 1;8(23):2857-67. doi: 10.1101/gad.8.23.2857.
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Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11909-13. doi: 10.1073/pnas.91.25.11909.
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