Johnson L, Mckenzie K S, Snell J R
Department of Veterinary Anatomy and Public Health, College Veterinary Medicine, Faculty of Toxicology, Texas A&M University, College Station 77843-4458, USA.
Tissue Cell. 1996 Apr;28(2):127-36. doi: 10.1016/s0040-8166(96)80001-2.
Serial cross sections were evaluated to determine the architectural arrangement of stages among men with varied spermatogenic efficiencies. Using autopsy specimens, glutaraldehyde-perfused testes from men with low or high daily sperm production per g parenchyma were compared. Lobes of testicular parenchyma were teased from connective tissue septa, further fixed in osmium, and embedded such that the straight portions of tubules could be sectioned perpendicularly. Unstained 22 microns serial sections were sectioned optically with Nomarski optics. Paired photomicrographs of each tubular cross section were taken under a 40 x objective, and stages of the spermatogenic cycle were mapped by two observers using Clermont's criteria (Clermont, 1963). For comparison, numbers (1-6) were assigned randomly to the stages, the stages were plotted in two dimensions (length and circumference of tubule) as if the tubule were cut down its length and laid flat, and geometric centers were plotted for each stage. Geometric centers consecutive and/or non-consecutive stages appeared to form an angle down the length of the tubule. When considering helical patterns along the tubule, men with neither low nor high spermatogenic efficiency had a complete wave composed of all six consecutive stages. The helical pattern of geometric centers indicated only 2-4 consecutive stages when the actual values of stages were used or when random numbers were substituted for actual numerical value of stages. The number of consecutive stages in tubules from these men was not different from consecutiveness found when stages were assigned random numbers. Given that no complete wave was found, regardless of spermatogenic efficiency, and that the degree of consecutiveness of stages down a helical pattern in human seminiferous tubules could be generated from random numbers, the arrangement of stages in human seminiferous tubules may simply be a random occurrence.
对连续的横断面进行评估，以确定不同生精效率男性各阶段的结构排列。使用尸检标本，比较了每克实质每日精子产量低或高的男性经戊二醛灌注的睾丸。从结缔组织隔中分离出睾丸实质叶，进一步用锇固定，并进行包埋，以便能垂直切取小管的直部。用诺马斯基光学显微镜光学切片，得到22微米厚的未染色连续切片。在40倍物镜下拍摄每个小管横断面的配对显微照片，由两名观察者根据克莱蒙特标准（克莱蒙特，1963年）绘制生精周期的各阶段。为了进行比较，将数字（1 - 6）随机分配给各阶段，将这些阶段绘制在二维图上（小管的长度和周长），就好像将小管沿其长度切开并平铺一样，并为每个阶段绘制几何中心。连续和/或不连续阶段的几何中心似乎沿小管长度形成一个角度。当考虑沿小管的螺旋模式时，生精效率既不低也不高的男性有一个由所有六个连续阶段组成的完整波。当使用阶段的实际值或用随机数代替阶段的实际数值时，几何中心的螺旋模式仅显示2 - 4个连续阶段。这些男性小管中连续阶段的数量与随机分配阶段时发现的连续性没有差异。鉴于无论生精效率如何都未发现完整波，并且人类曲细精管中沿螺旋模式各阶段的连续程度可以由随机数产生，人类曲细精管中各阶段的排列可能只是随机发生的。
