Carlson G P, Olson R M
Department of Pharmacology and Toxicology School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, IN 47907-1334, USA.
Biochem Mol Biol Int. 1995 Sep;37(1):65-71.
The metabolism of 1-butanol, 1-pentanol and 1-propanol by rat hepatic and pulmonary cytosolic preparations was measured with regard to ADH activity as influenced by pH and substrate concentration. Compared to lung, hepatic ADH activity showed little pH dependence with apparent Vmax values similar for the 3 alcohols. Apparent Km values were also similar and were lower than previously reported for ethanol. In contrast to the liver, little ADH activity was observed in pulmonary preparations at pH 7.2 or 9.0 with any alcohol. Pulmonary apparent Km values were considerably higher than those in the liver. Thus the optimum conditions for pulmonary ADH activity require an alkaline pH and high substrate concentrations.
以pH值和底物浓度对乙醇脱氢酶（ADH）活性的影响为指标，测定了大鼠肝脏和肺胞质提取物对1-丁醇、1-戊醇和1-丙醇的代谢情况。与肺相比，肝脏ADH活性对pH值的依赖性较小，三种醇的表观Vmax值相似。表观Km值也相似，且低于先前报道的乙醇的Km值。与肝脏相反，在pH 7.2或9.0条件下，用任何一种醇处理时，肺提取物中几乎未观察到ADH活性。肺的表观Km值远高于肝脏中的表观Km值。因此，肺ADH活性的最佳条件需要碱性pH值和高底物浓度。
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