Tornero P, Conejero V, Vera P
Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica-Consejo Superior de Investigaciones Científicas, Valencia, Spain.
Plant J. 1996 May;9(5):639-48. doi: 10.1046/j.1365-313x.1996.9050639.x.
This paper reports the isolation and characterization of a homeobox gene (VAHOX1) from Lycopersicon esculentum encoding a homeodomain protein that contains a leucine zipper motif. The bipartite homeodomain-leucine zipper (HD-Zip) motif has only been found in homeobox genes from dicotyledonous plants, indicating that this type of transcription factor regulates particular developmental processes in these plant species. Here, the genomic organization, sequence comparison and expression analysis of VAHOX1 are described. Transcriptional fusion of the 5' promoter region of VAHOX1 with the reporter GUS gene (VAHOX1-GUS) and expression analysis of this construct in transgenic plants indicates that VAHOX1 is specifically expressed in the phloem during phases of secondary growth. Unlike other plant homeobox genes, VAHOX1 does not appear to be expressed in meristems, and the function of VAHOX1 is considered a likely candidate molecule that may participate in the regulation of the identity and/or activity of phloem tissues during secondary phases of vascular development.
本文报道了从番茄中分离和鉴定出一个同源异型框基因（VAHOX1），该基因编码一种含有亮氨酸拉链基序的同源异型结构域蛋白。二分体同源异型结构域 - 亮氨酸拉链（HD - Zip）基序仅在双子叶植物的同源异型框基因中发现，这表明这种类型的转录因子调节这些植物物种中的特定发育过程。在此，描述了VAHOX1的基因组结构、序列比较和表达分析。VAHOX1的5'启动子区域与报告基因GUS基因的转录融合（VAHOX1 - GUS）以及该构建体在转基因植物中的表达分析表明，VAHOX1在次生生长阶段在韧皮部中特异性表达。与其他植物同源异型框基因不同，VAHOX1似乎不在分生组织中表达，并且VAHOX1的功能被认为是一个可能参与维管发育次生阶段韧皮部组织特性和/或活性调节的候选分子。
