Engbaek K, Johansen K S, Jensen M E
J Clin Pathol. 1979 Feb;32(2):187-90. doi: 10.1136/jcp.32.2.187.
Five techniques for Gram staining bacteria in paraffin sections were compared on serial sections of pulmonary tissues from eight bacteriological necropsies. Brown and Hopp's method was the most satisfactory for distinguishing Gram-positive and Gram-negative bacteria. However, this method cannot be recommended as the preparations were frequently overstained, and the Gram-negative bacteria were stained indistinctly. A modification of Brown and Hopps' method was developed which stains larger numbers of Gram-negative bacteria and differentiates well between different cell types and connective tissue, and there is no risk of overstaining.
在来自八例细菌学尸检的肺组织连续切片上，对石蜡切片中细菌进行革兰氏染色的五种技术进行了比较。布朗和霍普斯方法在区分革兰氏阳性菌和革兰氏阴性菌方面最令人满意。然而，由于制片经常过度染色，革兰氏阴性菌染色不清晰，因此该方法不推荐使用。开发了一种布朗和霍普斯方法的改良方法，该方法能使更多革兰氏阴性菌染色，并能很好地区分不同细胞类型和结缔组织，且不存在过度染色的风险。
