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正常人类精液中非精子细胞中的RNA和蛋白质合成

RNA and protein synthesis in the nonspermatozoal cells of normal human semen.

作者信息

Williams M A, Smith D C

机构信息

Department of Biomedical Science, University of Sheffield, UK.

出版信息

J Anat. 1996 Feb;188 ( Pt 1)(Pt 1):137-47.

PMID:8655400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1167641/
Abstract

A series of semen samples from recently fertile men was obtained by masturbation. They were washed and placed in short term culture in BWW medium, and labelled either with tritiated uridine or tritiated tyrosine. Control samples were labelled in the presence of actinomycin D and puromycin respectively, and examined by light and electron microscopy. Ejaculated spermatocytes, spermatids of various stages and certain anucleate bodies proved to be labelled with tritiated tyrosine and hence to be protein synthetic. Ejaculated spermatocytes were also active in RNA synthesis. Macrophages and neutrophil leucocytes both heavily incorporated tritiated uridine and tritiated tyrosine. The significance of these results is discussed in relation to the possible role of exfoliated sperm precursors in ejaculates.

摘要

通过手淫获取了近期有生育能力男性的一系列精液样本。将这些样本洗涤后置于BWW培养基中进行短期培养，并用氚标记的尿苷或氚标记的酪氨酸进行标记。对照样本分别在放线菌素D和嘌呤霉素存在的情况下进行标记，并通过光学显微镜和电子显微镜检查。射精的精母细胞、不同阶段的精子细胞以及某些无核小体被证明用氚标记的酪氨酸进行了标记，因此具有蛋白质合成活性。射精的精母细胞在RNA合成方面也很活跃。巨噬细胞和中性粒细胞都大量掺入了氚标记的尿苷和氚标记的酪氨酸。结合射精中脱落的精子前体的可能作用，对这些结果的意义进行了讨论。

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本文引用的文献

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RIBONUCLEIC ACID SYNTHESIS DURING MITOSIS AND MEIOSIS IN THE MOUSE TESTIS.小鼠睾丸有丝分裂和减数分裂过程中的核糖核酸合成
J Cell Biol. 1964 Sep;22(3):521-32. doi: 10.1083/jcb.22.3.521.
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An improved approach to the analysis of autoradiographs containing isolated sources of simple shape: method, theoretical basis and reference data.一种用于分析包含简单形状孤立源的放射自显影片的改进方法：方法、理论基础和参考数据。
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Synthetic activities during spermatogenesis in the mouse RNA and protein.
小鼠精子发生过程中的合成活动：RNA与蛋白质
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Lysosome formation and surface changes in stimulated peritoneal cells.刺激腹膜细胞中溶酶体的形成及表面变化
Z Zellforsch Mikrosk Anat. 1968;89(3):328-54. doi: 10.1007/BF00319245.
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Do non-spermatozoal cells mainly stem from spermiogenesis? Study of 106 fertile and 102 subfertile men.非精子细胞主要源于精子发生过程吗？对106名生育能力正常和102名生育能力低下男性的研究。
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Leucocytes in the ejaculate from fertile and infertile men.
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The characterisation of non-sperm cells in the ejaculates of fertile men using transmission electron microscopy.利用透射电子显微镜对生育期男性精液中的非精子细胞进行表征。
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Functional significance of white blood cells in the male and female reproductive tract.白细胞在男性和女性生殖道中的功能意义。
Hum Reprod. 1990 Aug;5(6):639-48. doi: 10.1093/oxfordjournals.humrep.a137162.
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Cell Differ. 1977 Mar;5(5-6):343-55. doi: 10.1016/0045-6039(77)90072-0.