Müller D J, Baumeister W, Engel A
M. E. Miller-Institute for Microscopic Structural Biology, Biozentrum, University of Basel, Switzerland.
J Bacteriol. 1996 Jun;178(11):3025-30. doi: 10.1128/jb.178.11.3025-3030.1996.
Both surfaces of the hexagonally packed intermediate (HPI) layer of Deinococcus radiodurans were imaged in buffer solution by atomic force microscopy. When adsorbed to freshly cleaved mica, the hydrophilic outer surface of the HPI layer was attached to the substrate and the hydrophobic inner surface was exposed to the stylus. The height of a single HPI layer was 7.0 nm, while overlapping edges of adjacent single layers adsorbed to mica had a height of 14.7 nm. However, double-layered stacks with inner surfaces facing each other exhibited a height of 17.4 nm. These stacks exposed the outer surface to the stylus. The different heights of overlapping layers and stacks are attributed to differences in the interaction between inner and outer surfaces. At high resolution, the inner surface revealed a protruding core with a central pore connected by six emanating arms. The pores exhibited two conformations, one with and the other without a central plug. Individual pores were observed to switch from one state to the other.
利用原子力显微镜对耐辐射球菌的六角形堆积中间体（HPI）层的两个表面在缓冲溶液中进行成像。当吸附到新劈开的云母上时，HPI层的亲水性外表面附着在基底上，而疏水性内表面则暴露于探针。单个HPI层的高度为7.0纳米，而吸附到云母上的相邻单层的重叠边缘高度为14.7纳米。然而，内表面相互面对的双层堆叠高度为17.4纳米。这些堆叠将外表面暴露于探针。重叠层和堆叠的不同高度归因于内表面和外表面之间相互作用的差异。在高分辨率下，内表面显示出一个突出的核心，其中心孔由六个放射状臂连接。这些孔呈现出两种构象，一种有中心塞，另一种没有。观察到单个孔会从一种状态转变为另一种状态。
