鼠伤寒沙门氏菌渗透调节性proU启动子的定点突变分析。
Site-directed mutational analysis of the osmotically regulated proU promoter of Salmonella typhimurium.
作者信息
Zhang X, Fletcher S A, Csonka L N
机构信息
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
出版信息
J Bacteriol. 1996 Jun;178(11):3377-9. doi: 10.1128/jb.178.11.3377-3379.1996.
Abstract
We carried out PCR mutagenesis of the proU promoter of Salmonella typhimurium, in order to identify sequences important for its osmotic control. We obtained five mutations in the -35 element: two decreased the promoter strength, one increased it, and the others had no effect. However, none abolished osmotic control, suggesting that the sequence of the -35 element is not crucial for osmotic control.
摘要
我们对鼠伤寒沙门氏菌的proU启动子进行了PCR诱变，以确定对其渗透调控重要的序列。我们在-35元件中获得了五个突变：两个降低了启动子强度，一个提高了启动子强度，其他的则没有影响。然而，没有一个突变消除渗透调控，这表明-35元件的序列对于渗透调控并非至关重要。
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