枯草芽孢杆菌主要柠檬酸合酶的特性分析
Characterization of the major citrate synthase of Bacillus subtilis.
作者信息
Jin S, Sonenshein A L
机构信息
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
出版信息
J Bacteriol. 1996 Jun;178(12):3658-60. doi: 10.1128/jb.178.12.3658-3660.1996.
Abstract
The major citrate synthase of Bacillus subtilis (CS-II) was purified to near homogeneity and shown to correspond to the product of the citZ gene. Accumulation of CS-II during exponential growth and stationary phases paralleled expression of the citZ gene. The physical and kinetic properties of CS-II were similar to those of citrate synthase enzymes from Bacillus megaterium and from eukaryotic cells but differed from those of citrate synthases from many gram-negative bacteria.
摘要
枯草芽孢杆菌的主要柠檬酸合酶（CS-II）被纯化至近乎同质，并被证明与citZ基因的产物相对应。在指数生长期和稳定期CS-II的积累与citZ基因的表达平行。CS-II的物理和动力学特性与巨大芽孢杆菌及真核细胞中的柠檬酸合酶相似，但与许多革兰氏阴性菌中的柠檬酸合酶不同。
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本文引用的文献
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