Sekhon A S
Mycopathologia. 1977 Apr 29;60(3):145-9. doi: 10.1007/BF00448406.
Cultures of the + (UAMH 1485) and - (UAMH 1486) mating types of Nannizzia gypsea and their crosses (1485 x 1486) were analyzed, after 1, 2, 3, and 4 weeks growth, for fresh weight, proteins, peroxidases, esterases, acid- and alkaline-phosphatases. The + type produced slightly more growth at all periods (90, 131, 131, and 129 mg/plate) than the - mating type (90, 123, 121, and 108 mg/plate). The growth of the crosses was much less (30, 88, 83, and 94 mg/plate). Total soluble proteins per gram fresh weight was greater for the crosses (51, 33, 48, and 63 mg) than for the + (19, 32, 34, and 32 mg) and - (20, 33, 33, and 33 mg) strains. Disc-gel electrophoretic analyses revealed 6-9 protein bands in each of the mating types, and 7-8 bands (protein) in crosses, several of which showed mobility and stain uptake differences. There were 1-2 peroxidase bands in the +, -, and crosses; their patterns were generally the same in 1-week-old cultures, but slight differences were found at the subsequent harvests. The number of esterases in the +, -, and crosses was 3-4 and 2-3, respectively, but their patterns were similar. Acid- and alkaline-phosphatases of the + and -, and crosses were quite similar to one another. For all the enzymes studied, the number of bands increased after the first harvest in crosses, but in the single strains there was no constant pattern.
对燕麦色癣菌的 +（UAMH 1485）和 -（UAMH 1486）交配型及其杂交种（1485×1486）进行培养，在生长1、2、3和4周后，分析其鲜重、蛋白质、过氧化物酶、酯酶、酸性和碱性磷酸酶。在所有时期，+ 型（每平板90、131、131和129毫克）的生长量略高于 - 交配型（每平板90、123、121和108毫克）。杂交种的生长量要少得多（每平板30、88、83和94毫克）。每克鲜重的总可溶性蛋白质，杂交种（51、33、48和63毫克）比 + 菌株（19、32、34和32毫克）和 - 菌株（20、33、33和33毫克）更多。圆盘凝胶电泳分析显示，每种交配型中有6 - 9条蛋白质带，杂交种中有7 - 8条（蛋白质）带，其中几条带在迁移率和染色吸收上存在差异。+、 - 和杂交种中有1 - 2条过氧化物酶带；在1周龄培养物中它们的模式通常相同，但在随后的收获期发现了细微差异。+、 - 和杂交种中酯酶的数量分别为3 - 4条和2 - 3条，但它们的模式相似。+、 - 和杂交种的酸性和碱性磷酸酶彼此非常相似。对于所有研究的酶，杂交种在第一次收获后条带数量增加，但单一菌株中没有固定模式。
