Ludes B, Coste M, Tracqui A, Mangin P
Institut de Médecine Légale, Strasbourg, France.
J Forensic Sci. 1996 May;41(3):425-8.
The diagnosis of drowning is one of the most difficult in forensic pathology. Diatom analysis has been proposed to provide supportive evidence of drowning but the reliability and applicability of quantitative and qualitative diatom analysis in the diagnosis of drowning is still disputed in the literature. In order to further examine such cases, the authors report the development of a water monitoring system based on algae performed each month at three aquatic locations where drowning victims are frequently found. Water samples and stones were taken from the surface and from the bed of the river. This protocol was performed during 1993 with analyses both on water samples and human tissue samples (30 bodies). The diatom profile of the drowning sites was compared with the tissue analysis. The extraction of diatoms from the tissues was performed with an enzymatic digestion method using Proteinase K. Results indicate that the monitoring of river diatom populations is an accurate method of generating profiles of the river flora, which can then be compared with the diatom genera found in tissues.
溺水的诊断是法医病理学中最困难的诊断之一。硅藻分析已被提出来为溺水提供支持性证据，但定量和定性硅藻分析在溺水诊断中的可靠性和适用性在文献中仍存在争议。为了进一步研究此类案例，作者报告了一种基于藻类的水监测系统的开发情况，该系统每月在三个经常发现溺水受害者的水域地点进行。从河流表面和河床采集水样和石头。该方案在1993年实施，对水样和人体组织样本（30具尸体）都进行了分析。将溺水地点的硅藻图谱与组织分析进行比较。使用蛋白酶K通过酶消化法从组织中提取硅藻。结果表明，监测河流硅藻种群是生成河流植物群图谱的准确方法，然后可以将其与组织中发现的硅藻属进行比较。
