胸腺细胞中Bc1-3的组成型表达增加了体内NF-κB1（p50）同二聚体的DNA结合。
Constitutive expression of Bc1-3 in thymocytes increases the DNA binding of NF-kappaB1 (p50) homodimers in vivo.
作者信息
Caamaño J H, Perez P, Lira S A, Bravo R
机构信息
Department of Oncology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000, USA.
出版信息
Mol Cell Biol. 1996 Apr;16(4):1342-8. doi: 10.1128/MCB.16.4.1342.
Abstract
Previous studies have indicated that Bcl-3 interacts through its ankyrin repeats with the transcriptional factors NF-kappaB1 (p50) and NF-kappaB2 (p52), affecting their biological activities. To further investigate the role of Bcl-3 in vivo and its association with the NF-kappaB proteins, we have generated transgenic mice constitutively expressing Bcl-3 in thymocytes. The results indicate that Bcl-3 is associated with endogenous p50 and p52 in nuclear extracts from transgenic animals. Remarkably, constitutive expression of Bcl-3 in these cells augments the DNA binding activity of p52 homodimers. This effect could be reproduced in vitro and is blocked by anti-Bcl-3 antibodies. We have also shown that Bcl-3 is phosphorylated in thymocytes and that its dephosphorylation greatly decreases the effect on p50 homodimers.
摘要
先前的研究表明，Bcl-3通过其锚蛋白重复序列与转录因子NF-κB1（p50）和NF-κB2（p52）相互作用，影响它们的生物学活性。为了进一步研究Bcl-3在体内的作用及其与NF-κB蛋白的关联，我们构建了在胸腺细胞中组成性表达Bcl-3的转基因小鼠。结果表明，在转基因动物的核提取物中，Bcl-3与内源性p50和p52相关联。值得注意的是，这些细胞中Bcl-3的组成性表达增强了p52同二聚体的DNA结合活性。这种效应在体外可以重现，并且被抗Bcl-3抗体阻断。我们还表明，Bcl-3在胸腺细胞中被磷酸化，其去磷酸化大大降低了对p50同二聚体的影响。
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