Schickel J, Helmig C, Meinhardt F
Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Germany.
Nucleic Acids Res. 1996 May 15;24(10):1879-86. doi: 10.1093/nar/24.10.1879.
All of the 14 genes encoded by the cytoplasmic linear killer plasmids of Kluyveromyces lactis are preceded by upstream conserved sequences (UCSs), cis-acting elements involved in plasmid gene transcription. Using the bacterial glucose-dehydrogenase gene as a reporter, expression driven by seven cytoplasmic promoters was determined. The level of expression ranged from 0.5 to 6 nkat. The highest activity was displayed by UCS 6 of pGKL2 whereas the lowest level was obtained with UCS2 of pGKL2, all other values were in between. Sequences located 5' upstream the UCSs do not influence expression. As exemplified for UCS5 and UCS10, deletion led to an almost complete loss of expression.
乳酸克鲁维酵母胞质线性杀伤质粒编码的所有14个基因之前都有上游保守序列(UCSs),这是参与质粒基因转录的顺式作用元件。以细菌葡萄糖脱氢酶基因作为报告基因,测定了7个胞质启动子驱动的表达。表达水平在0.5至6纳卡特之间。pGKL2的UCS 6表现出最高活性,而pGKL2的UCS2活性最低,所有其他值介于两者之间。位于UCSs 5'上游的序列不影响表达。以UCS5和UCS10为例,缺失导致表达几乎完全丧失。