Stark M J, Mileham A J, Romanos M A, Boyd A
Nucleic Acids Res. 1984 Aug 10;12(15):6011-30. doi: 10.1093/nar/12.15.6011.
In killer strains of the yeast Kluyveromyces lactis, production of a protein toxin which inhibits the growth of sensitive yeast cells is associated with the presence of two linear DNA plasmids, k1 and k2. We have determined the nucleotide sequence of the smaller plasmid k1 (8.9kb) which is thought to carry the structural gene(s) encoding the toxin. The plasmid has a low G + C content (26.8%) and contains four long open reading frames which account for over 95% of the total sequence. The longest open reading frame (1146 amino acids) probably corresponds to a structural gene for the killer toxin. Transcripts from three of the putative genes have been detected in K.lactis by Northern hybridisation.
在乳酸克鲁维酵母的杀伤菌株中，一种抑制敏感酵母细胞生长的蛋白质毒素的产生与两个线性DNA质粒k1和k2的存在有关。我们已经确定了较小的质粒k1（8.9kb）的核苷酸序列，该质粒被认为携带编码毒素的结构基因。该质粒的G + C含量较低（26.8%），包含四个长开放阅读框，占总序列的95%以上。最长的开放阅读框（1146个氨基酸）可能对应于杀伤毒素的结构基因。通过Northern杂交在乳酸克鲁维酵母中检测到了三个推定基因的转录本。
