Langlois R G, Jensen R H
J Histochem Cytochem. 1979 Jan;27(1):72-9. doi: 10.1177/27.1.86583.
The interactions between DNA-specific fluorescence stains complexed with mitotic Chinese hamster cells were studied by spectrofluorometric and flow fluorometric techniques. The degree of binding interactions and of energy transfer between stains was determined from the intensities and shapes of fluorescence emission spectra of cells complexed with pairs of stains. The stain pairs Hoechst 33258-chromomycin A3, Hoechst 33258-ethidium bromide, and chromomycin A3-ethidium bromide exhibited efficient energy transfer from the short wavelength absorber (donor) to the long wavelength absorber (acceptor), and little competitive or cooperative binding of stains. The stain pair quinacrine-ethidium bromide exhibited both energy transfer and competitive binding. None of the stain pairs showed evidence of strong electronic interactions between stains. The magnitude of energy transfer interactions was used to estimate the quantity and distribution of the stains molecules complexed to mitotic cells. The results indicate a fairly even distribution of each of these stains along the DNA of intracellular mitotic chromosomes.
采用荧光分光光度法和流式荧光测定技术，研究了与有丝分裂期中国仓鼠细胞复合的DNA特异性荧光染料之间的相互作用。根据与成对染料复合的细胞荧光发射光谱的强度和形状，确定了染料之间的结合相互作用程度和能量转移程度。染料对Hoechst 33258-放线菌素A3、Hoechst 33258-溴化乙锭和放线菌素A3-溴化乙锭表现出从短波长吸收剂（供体）到长波长吸收剂（受体）的有效能量转移，且染料的竞争性或协同结合很少。染料对喹吖因-溴化乙锭既表现出能量转移又表现出竞争性结合。没有一对染料显示出染料之间存在强电子相互作用的证据。能量转移相互作用的大小用于估计与有丝分裂细胞复合的染料分子的数量和分布。结果表明，这些染料中的每一种沿着细胞内有丝分裂染色体的DNA分布相当均匀。
