白细胞介素-1β转化酶样蛋白酶的切割位点定向抑制剂可抑制大鼠原代肝细胞培养中的细胞凋亡。
A cleavage-site-directed inhibitor of interleukin-1 beta-converting enzyme-like proteases inhibits apoptosis in primary cultures of rat hepatocytes.
作者信息
Cain K, Inayat-Hussain S H, Couet C, Cohen G M
机构信息
MRC Toxicology Unit, Leicester, U.K.
出版信息
Biochem J. 1996 Feb 15;314 ( Pt 1)(Pt 1):27-32. doi: 10.1042/bj3140027.
Abstract
Apoptosis induced in primary hepatocytes by transforming growth factor beta1 and staurosporine produced chromatin condensation, DNA cleavage is detected by in situ end-labelling, field inversion and conventional gel electrophoresis, and cell detachment. These effects were abolished by benzyloxycarbonyl-valinylalanylaspartylfluoromethyl ketone, a cleavage-site-directed inhibitor of interleukin-1beta-converting enzyme-like proteases, and this finding suggests that these enzymes are involved in liver apoptosis.
摘要
转化生长因子β1和星形孢菌素在原代肝细胞中诱导的细胞凋亡产生了染色质浓缩，通过原位末端标记、脉冲场凝胶电泳和常规凝胶电泳检测到DNA裂解以及细胞脱离。这些效应被苄氧羰基-缬氨酰丙氨酰天冬氨酰氟甲基酮（一种白细胞介素-1β转化酶样蛋白酶的切割位点定向抑制剂）消除，这一发现表明这些酶参与了肝脏细胞凋亡。
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本文引用的文献
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Condensation of the chromatin at the membrane of an apoptotic nucleus is not associated with activation of an endonuclease.凋亡细胞核膜处染色质的凝聚与核酸内切酶的激活无关。
J Cell Sci. 1993 Feb;104 ( Pt 2):317-26. doi: 10.1242/jcs.104.2.317.
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Apoptosis: the biochemistry and molecular biology of programmed cell death.细胞凋亡：程序性细胞死亡的生物化学与分子生物学
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The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme.秀丽隐杆线虫细胞死亡基因ced-3编码一种与哺乳动物白细胞介素-1β转化酶相似的蛋白质。
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Role of proteolysis in apoptosis: involvement of serine proteases in internucleosomal DNA fragmentation in immature thymocytes.蛋白水解在细胞凋亡中的作用：丝氨酸蛋白酶参与未成熟胸腺细胞的核小体间DNA片段化过程。
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Calpain activation in apoptosis.细胞凋亡中的钙蛋白酶激活
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P1 aspartate-based peptide alpha-((2,6-dichlorobenzoyl)oxy)methyl ketones as potent time-dependent inhibitors of interleukin-1 beta-converting enzyme.基于天冬氨酸的肽α-((2,6-二氯苯甲酰基)氧基)甲基酮作为白细胞介素-1β转化酶的有效时间依赖性抑制剂。
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Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE.一种具有类ICE特性的蛋白酶对聚（ADP - 核糖）聚合酶的切割作用。
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