苄氧羰基-缬氨酸-丙氨酸-天冬氨酸（甲酯）氟甲基酮（Z-VAD.FMK）通过阻断CPP32的加工过程来抑制细胞凋亡。
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32.
作者信息
Slee E A, Zhu H, Chow S C, MacFarlane M, Nicholson D W, Cohen G M
机构信息
MRC Toxicology Unit, University of Leicester, Lancaster Road, Leicester, U.K.
出版信息
Biochem J. 1996 Apr 1;315 ( Pt 1)(Pt 1):21-4. doi: 10.1042/bj3150021.
Abstract
Interleukin-1 beta converting enzyme (ICE)-like proteases, which are synthesized as inactive precursors, play a key role in the induction of apoptosis. We now demonstrate that benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK), an ICE-like protease inhibitor, inhibits apoptosis by preventing the processing of CPP32 to its active form. These results suggest that novel inhibitors of apoptosis can be developed which prevent processing of proforms of ICE-like proteases.
摘要
白细胞介素-1β转化酶（ICE）样蛋白酶以无活性前体形式合成，在细胞凋亡诱导中起关键作用。我们现在证明，苄氧羰基-Val-Ala-Asp（OMe）氟甲基酮（Z-VAD.FMK），一种ICE样蛋白酶抑制剂，通过阻止CPP32加工成其活性形式来抑制细胞凋亡。这些结果表明，可以开发新型凋亡抑制剂，其可阻止ICE样蛋白酶前体形式的加工。
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A cleavage-site-directed inhibitor of interleukin-1 beta-converting enzyme-like proteases inhibits apoptosis in primary cultures of rat hepatocytes.白细胞介素-1β转化酶样蛋白酶的切割位点定向抑制剂可抑制大鼠原代肝细胞培养中的细胞凋亡。
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