Inoue A, Otsuka E, Hiruma Y, Hirose S, Furuya M, Tanaka S, Hagiwara H
Research Center for Experimental Biology, Tokyo Institute of Technology, Yokohama, Japan.
Biochem Biophys Res Commun. 1996 Nov 1;228(1):182-6. doi: 10.1006/bbrc.1996.1636.
The effects were examined of treatment with retinoids of osteoblastic MC3T3-E1 cells on the natriuretic peptide system that promotes the differentiation of osteoblastic cells. Northern blot analysis revealed high levels of mRNA for the retinoid X receptor beta (RXR beta) and moderate levels of mRNAs for retinoic acid receptors alpha (RAR alpha) and gamma (RAR gamma). Exposure of MC3T3-E1 cells to 1 microM retinoid caused increases in the levels of C-type natriuretic peptide (CNP) and natriuretic peptide receptor-C (NPR-C). The activity of natriuretic peptide receptor-B (NPR-B) was unchanged after the addition of retinoid to the culture system. These results suggest that retinoids might influence the metabolism of osteoblastic cells through regulation of the natriuretic peptide system.
研究了用类视黄醇处理成骨细胞MC3T3-E1细胞对促进成骨细胞分化的利钠肽系统的影响。Northern印迹分析显示，类视黄醇X受体β（RXRβ）的mRNA水平较高，而维甲酸受体α（RARα）和γ（RARγ）的mRNA水平中等。将MC3T3-E1细胞暴露于1 microM类视黄醇会导致C型利钠肽（CNP）和利钠肽受体-C（NPR-C）水平升高。向培养系统中添加类视黄醇后，利钠肽受体-B（NPR-B）的活性未发生变化。这些结果表明，类视黄醇可能通过调节利钠肽系统来影响成骨细胞的代谢。
