Klingler M, Soong J, Butler B, Gergen J P
Zoologisches Institut, Luisenstrasse 14, Munich, D-80333, Germany.
Dev Biol. 1996 Jul 10;177(1):73-84. doi: 10.1006/dbio.1996.0146.
The segmented body pattern of the Drosophila embryo is established through a hierarchical network of interacting genes. At each successive step in this pathway, transcriptional regulation is used to convert coarse positional information into finer patterns of gene expression. Central to this process are the cis-regulatory regions that drive the dynamic spatial expression of the different segmentation genes. Here we describe the cis-regulatory region of the runt gene. As found for both other primary pair-rule genes, hairy and even-skipped, there are stripe-specific elements which mediate the initial regulation of runt stripes by gap genes. We did not find autoregulatory elements as described for even-skipped and fushi tarazu. The regulation of runt by other pair-rule genes is mediated by a large region, extending over 5 kb upstream and downstream of the transcription start site. This "disperse" element cannot be subdivided into functionally independent subelements or minimal elements. Such disperse elements mediating pair-rule gene interactions may have escaped detection in other segmentation genes and may involve molecular mechanisms different from those mediating regulation by gap genes.
果蝇胚胎的分节身体模式是通过一个相互作用基因的层次网络建立的。在该途径的每一个连续步骤中，转录调控被用于将粗略的位置信息转化为更精细的基因表达模式。这个过程的核心是驱动不同分节基因动态空间表达的顺式调控区域。在这里，我们描述了矮胖基因的顺式调控区域。正如在其他两个主要的成对规则基因（毛状基因和偶数缺失基因）中所发现的那样，存在条纹特异性元件，它们介导间隙基因对矮胖条纹的初始调控。我们没有发现像偶数缺失基因和分节基因那样描述的自调控元件。其他成对规则基因对矮胖基因的调控是由一个大区域介导的，该区域在转录起始位点上游和下游延伸超过5 kb。这种“分散”元件不能细分为功能上独立的子元件或最小元件。这种介导成对规则基因相互作用的分散元件可能在其他分节基因中未被检测到，并且可能涉及与介导间隙基因调控的分子机制不同的分子机制。
