Jackson M S, See C G, Mulligan L M, Lauffart B F
Department of Human Genetics, University of Newcastle upon Tyne, United Kingdom.
Genomics. 1996 Apr 15;33(2):258-70. doi: 10.1006/geno.1996.0190.
We present a yeast artificial chromosome (YAC) and pulsed-field gel electrophoresis (PFGE) map across the centromere of human chromosome 10 that links expressed sequences in 10p11 to expressed sequences in 10q11.2. This map is the first of its kind to link genes across a human centromere. It consists of a 2.5-Mb YAC contig extending from 10p11 to our previously published 5.35-Mb PFGE map of the centromeric satellite arrays, and a 2.65-Mb YAC contig extending from these satellite arrays to 10q11.2. This map covers approximately 6.5-7% of the total DNA of chromosome 10. Two Généthon genetic markers, D10S578 and D10S604, are included. These markers are only 1 cM apart but are separated by a physical distance of more than 9.2 Mb, including the centromere. This gives a ratio of genetic to physical distance of 0.11 cM/Mb, 9-11 times lower than average estimates for the human genome and chromosome 10. Markers linked to the centromere include the duplicated zinc finger genes ZNF11A, ZNF33A, and ZNF37A (which map to 10p11) and ZNF11B, ZNF33B, and ZNF37B (which map to 10q11.2). Restriction mapping confirms that the genes on each arm lie in opposite orientation with respect to the centromere, consistent with the hypothesis that a pericentric inversion has occurred in this region during primate evolution.
我们展示了一张跨越人类10号染色体着丝粒的酵母人工染色体（YAC）和脉冲场凝胶电泳（PFGE）图谱，该图谱将10p11中的表达序列与10q11.2中的表达序列联系起来。这是首张跨越人类着丝粒连接基因的此类图谱。它由一个从10p11延伸至我们之前发表的着丝粒卫星阵列5.35 Mb PFGE图谱的2.5 Mb YAC重叠群，以及一个从这些卫星阵列延伸至10q11.2的2.65 Mb YAC重叠群组成。该图谱覆盖了10号染色体总DNA的约6.5 - 7%。包含两个Généthon遗传标记D10S578和D10S604。这些标记在遗传距离上仅相距1 cM，但在物理距离上却被超过9.2 Mb分隔开，包括着丝粒。这使得遗传距离与物理距离的比率为0.11 cM/Mb，比人类基因组和10号染色体的平均估计值低9 - 11倍。与着丝粒相连的标记包括重复的锌指基因ZNF11A、ZNF33A和ZNF37A（定位于10p11）以及ZNF11B、ZNF33B和ZNF37B（定位于10q11.2）。限制性图谱分析证实每条臂上的基因相对于着丝粒呈相反方向排列，这与灵长类进化过程中该区域发生了臂间倒位的假说一致。
